六味地黃湯이 흰쥐의 腦挫傷 회복에 미치는 影響
Effects of yukmijihwangtang to recover the brain damage of mice
초록/요약
The aim of this study was to investigate the effects of Yukmijiwhangtang on contusion of the mice induced with medical stress. I observed its effects on the impaired tissue of brain using light and electron microscopes, and examined hematological changes and VEGF-immunohistochemistry. Hematological change : Leukocytes were increased significantly in the control group mice compared with experimental group, but erythrocytes, hematocrit and platelets did not occurred difference between control and experimental group. Light microscopical observation : Nuclei of neurons were condensed remarkedly in the 3-day control group, but few of nucleus were condensed in the 7-day experimental group. The most number of glial cells were observed in the 3-day experimental group compared with any other groups. Edema and dilatation of vessels occurred significantly in the 3-day control group, and these results occurred the most weakly in the 7-day experimental group. Examination for VEGF-immunohistochemistry : VEGF-immunohistochemical reactivity for the glial cells were the highest in the 3-day experimental group, and immunoreactivity for the vessels and neurons were the most increased in the 7-day experimental group. Electron microscopical observation : In the 3-day control group, protoplasmic astrocytes which concerned with angiogenesis were contained weakly developed rough endoplasmic reticulum, and a few of glial filaments were observed. In the 7-day experimental group, the bundles of glial filaments were found in the cytoplasmic process of astrocytes which bound vessels. In conclusion, medication Yukmijiwhangtang to mice contused by medical stress is highly effective in inflamatory response, curing cell damages and angiogenesis.
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ABSTRACT
I. 緖論 = 1
II. 材料 및 方法 = 2
1) 動物 = 2
2) 藥物 = 2
3) 腦挫傷誘發 = 2
4) 藥物의 投與 = 3
5) 體重의 測定 = 3
6) 血液學的 變化 = 4
7) Vascular Endothelial Growth Factor(VEGF)의 免疫組織化學的 檢査 = 4
8) 전자현미경 관찰 = 5
9) 統計處理 = 5
III. 結果 = 6
IV. 考察 = 22
V. 結論 = 28
參考文獻 = 29