祛風導痰湯이 白鼠의 腦血流 變化에 미치는 機轉硏究
A Mechanism Study of GeopungDodamTang on the Change of Cerebral Blood Flow in Rats
초록/요약
GeopungDodamTang(GDT) have been used in oriental medicine for many centuries as a therapeutic agent of apoplexy. The mechanism of GDT on the cerebral blood flow is not known. The purpose of this Study was to investigate effects of GDT on the pial arterial diameter and action mechanism of GDT-induced increased regional cerebral blood flow(rCBF) The changes of regional cerebral blood flow(rCBF) was determinated by Laser-Doppler Flowmetry(LDF), and the changes of pial arterial diameter were determinated by video microscopy methods and video analyzer. The results were as follows ; 1. Pial arterial diameter was significantly increased by GDT in a dose-dependent manner. 2. Pretreatment with L-NNA significantly inhibited GDT induced increased rCBF. 3. Pretreatment with methylene blue significantly inhibited GDT induced increased rCBF. 4. Pretreatment with indomethacin inhibited GDT induced increased rCBF. These results suggest that GDT causes a diverse response of cerebral hemodynamics(rCBF and pial arterial diameter). The cerebral hemodynamics is also mediated by nitric oxide synthase, cyclic GMP(guanylyl cyclase) and prostaglandin(cyclooxygenase).
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ABSTRACT
I. 緖論 = 1
II. 實驗材料 및 方法 = 3
1. 材料 = 3
2. 方法 = 3
3. 統計處理 = 5
III. 實驗成績 = 6
1. 祛風導痰湯이 腦軟膜動脈의 直徑에 미치는 變動效果 = 6
2. NOS 抑制劑 前處置 後 祛風導痰湯이 局所腦血流量에 미치는 作用機轉 = 7
3. Guanylyl cyclase 抑制劑 前處置 後 祛風導痰湯이 局所腦血流量에 미치는 作用機轉 = 8
4. Cyclooxygenase 抑制劑 前處置 後 祛風導痰湯이 局所腦血流量에 미치는 作用機轉 = 9
IV. 考察 = 10
V. 結論 = 14
參考文獻 = 15