理氣祛風散이 白鼠의 神經病理性 疼痛 抑制 및 c-Fos 蛋白 發顯에 미치는 影響
Effects of oral administration of ligigeopoongsan on neuropathic pain and c-Fos protein expression in rats
초록/요약
Objective : We have studied to know effects of Ligigeopoongsan(LGS, 理氣祛風散) on mechanical allodynia, cold allodynia and c-Fos protain expression in the model of neuropathic pain of rats. Methods : The model of neuropathic pain was made by injured tibial nerve and sural nerve while common peroneal nerve was maintained. After 2weeks, we performed behavioral tests for 7 days to try out mechanical allodynia using von frey filament and cold allodynia using acetone, which are calculated by counting withdrawal response on foot. Rat brains removed and sliced on 8th days. Serial sections were immunohistochemically reacted with polyclonal c-Fos antibody. The numbers of c-Fos protein immunoreactive neurons in the central gray were examined using scion image program. Results : 1. Mechanical allodynia in LGS-2, LGS-3 groups were significantly diminished compared with the control group. 2. Cold allodynia in LGS-3 group was dsignificantly iminished compared with the control group. 3. c-Fos protein expression on the central gray LGS-2, LGS-3 groups were significantly lower than that of control group. Conclusions : We have noticed that LGS(理氣祛風散) diminished mechnical and cold allodynia in the model of neuropathic pain compared with the control group. c-Fos protein expression in the central gray of that group was also decreased compared with the control group. Pain control group were LGS was accumulated as time goes by. This study can be used as a basic resource on a study and a treatment of pain.
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目次 = i
ABSTRACT = i
Ⅰ. 緖論 = 1
Ⅱ. 材料 및 方法 = 3
1. 실험재료 = 3
1) 동물 = 3
2) 약재 = 3
2. 실험방법 = 4
1) 검액의 조제 = 4
2) 신경병리성 동통 유발 및 군 분리 = 5
3) 경구투여 = 5
4) Von frey filament 및 Acetone 자극으로 유발된 異質痛 측정 = 5
5) c-Fos 단백 염색 = 6
3. 統計處理 = 7
Ⅲ. 結果 = 8
1. 理氣祛風散구강투여가 Von frey filament 자극으로 유발된 異質痛에 미치는 효과 = 8
2. 理氣祛風散구강 투여가 Acetone 자극으로 유발된 異質痛에 미치는 효과 = 10
3. 理氣祛風散구강투여가 c-Fos 단백 발현에 미치는 효과 = 12
Ⅳ. 考察 = 14
Ⅴ. 結論 = 22
參考文獻 = 23