蓮根 抽出物이 腦虛血 抑制 效果에 관한 實驗的 硏究
(An) Experimental study on the inhibitive effects of nelumbinis rhizomatis nodus extract on brain ischemia
초록/요약
This Study was designed to investigate the effects of Nelumbinis Rhizomatis Nodus (NRN) on the change of cerebral hemodynamics〔regional cerebral blood flow (rCBF), pial arterial diameter (PAD) and mean arterial blood pressure (MABP)〕in normal and cerebral ischemic rats, and further to determine the mechanisms of action of NRN. And, this study was designed to investigate the inhibition of lactate dehydrogenase activity in neuronal cells and cytokines production in serum of cerebral ischemic rats. The results were as follows: 1. NRN significantly increased rCBF and PAD in a dose-dependent manner, and NRN increased MABP in a dose-dependent manner. This results suggested that NRN significantly increased rCBF by dilating PAD. 2. The NRN-induced increase in rCBF was significantly inhibited by pretreatment with indomethacin (1 ㎎/㎏, i.p.), an inhibitor of cyclooxygenase as well as methylene blue (10 ㎍/㎏, i.p.), an inhibitor of guanylate cyclase. 3. The NRN-induced dilation in PAD was significantly inhibited by pretreatment with methylene blue and was inhibited by pretreatment with indomethacin. 4. The NRN-induced increase in MABP was reduced by pretreatment with indomethacin and methylene blue. This results suggested that the mechanism of action of NRN was mediated by guanylate cyclase. 5. Both rCBF and PAD were significantly and stably increased by NRN (10 ㎎/㎏, i.p.) during the period of cerebral reperfusion, which contrasted with the findings of rapid and marked increase in control group. 6. NRN significantly inhibited lactate dehydrogenase activity in neuronal cells. This results suggested that NRN prevented the neuronal death. 7. In cytokine production in the serum drawn from femoral arterial 1 hr after middle cerebral artery occlusion, sample group showed significantly decreased production of IL-1β and increased production of IL-10 and TGF-β compared with control group. 8. In cytokine production in the serum drawn from femoral arterial 1 hr after reperfusion, sample group showed significantly decreased production of IL-1β and TNF-α as well as increased production of IL-10 and TGF-β compared with control group. These results suggested that NRN had inhibitive effect on the brain damage by inhibiting IL-1β and TNF-α production, and by accelerating IL-10 and TGF-β production. Taken together, it is suggested that NRN had an anti-ischemic effect through the improvement of cerebral hemodynamics and inhibitive effect on the brain damage.
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目次
ABSTRACT = i
Ⅰ. 서론 = 1
Ⅱ. 재료 및 방법 = 2
1. 재료 = 2
1) 동물 = 2
2) 약재 = 2
2. 방법 = 2
1) 검액의 조제 = 2
2) 정상 흰쥐의 뇌혈류역학 변화 관찰 = 2
3) 정상 흰쥐의 뇌혈류역학 변화에 미치는 기전 관찰 = 4
4) 뇌허혈 병태모델의 뇌혈류역학 변동에 미치는 개선 효과 관찰 = 5
5) 생쥐의 신경 세포내 lactate dehydrogenase 활성도 측정 = 5
6) 뇌허혈 병태 모델의 혈청에서 생성되는 사이토카인 양 측정 = 6
3. 통계처리 = 9
Ⅲ. 실험성적 = 10
1. NRN이 정상 흰쥐의 뇌혈류역학에 미치는 효과 = 10
2. NRN 투여로 변화된 정상 흰쥐의 뇌혈류역학 작용기전 관찰 = 12
3. NRN이 뇌허혈 병태 모델의 생리학적 변수에 미치는 영향 = 18
4. NRN이 뇌허혈 병태 모델의 뇌혈류역학 변동에 미치는 개선 효과 = 19
5. NRN이 생쥐의 신경 세포내 LDH 활성도에 미치는 효과 = 23
6. NRN이 뇌허혈 병태 모델의 혈청에서 생성되는 사이토카인 양에 미치는 효과 = 25
Ⅳ. 고찰 = 29
Ⅴ. 결론 = 35
참고문헌 = 37