八物湯이 腦虛血 抑制效果에 미치는 實驗的 硏究
(The) Experimental study of Palmul-Tang against inhibitive effects on the brain ischemia
초록/요약
The Study was designed to investigate the effects of Palmul-Tang(PMT) on the change of cerebral hemodynamics〔regional cerebral blood flow(rCBF), pial arterial diameter(PAD) and mean arterial blood pressure(MABP)〕in normal and cerebral ischemic rats, and further to determine the mechanism of action of PMT. And, this Study was designed to investigate whether PMT inhibit lactate dehydrogenase activity in neuronal cells and cytokines production in serum of cerebral ischemic rats. The results in normal rats were as follows ; 1. PMT significantly increased rCBF and PAD in a dose-dependent, and PMT increased MABP in a dose-dependent. This results were suggested that PMT significantly increased rCBF by dilating PAD. 2. The PMT-induced increase in rCBF was significantly inhibited by pretreatment with indomethacin(1 ㎎/㎏, i.p.), an inhibitor of cyclooxygenase, and was inhibited by methylene blue(10 ㎍/㎏, i.p.), an inhibitor of guanylate cyclase. 3. The PMT-induced dilation in PAD was significantly inhibited by pretreatment with indomethacin and methylene blue. 4. The PMT-induced increase in MABP was significantly inhibited by pretreatment with indomethacin but was increased by methylene blue. This results were suggested that the mechanism of PMT was mediated by cyclooxygenase. 5. PMT significantly inhibited lactate dehydrogenase activity in neuronal cells. This results were suggested that PMT prevented the neuronal death. The results in cerebral ischemic rats were as follows ; 1. Both rCBF and PAD were significantly and stably increased by PMT(10 ㎎/㎏, i.p.) during the period of cerebral reperfusion, which contrasted with the findings of rapid and marked increase in control group. 2. In cytokine production of serum by drawing from femoral arterial blood after middle cerebral arterial occlusion 1 hr, sample group was decreased IL-1β and TNF-α production compared with control group, IL-10 production of sample group was similar to that of control group, but sample group was significantly increased TGF-β production compared with control group. 3. In cytokine production of serum by drawing from femoral arterial blood after reperfusion 1 hr, sample group was significantly decreased IL-1β production compared with control group and decreased TNF-α production compared with control group. IL-10 production of sample group was similar to that of control group, but sample group was significantly increased TGF-β production compared with control group. 4. In cytokine production of serum by drawing from femoral arterial blood after reperfusion 4 hrs, sample group was significantly decreased IL-1β production compared with control group, but IL-10 production of sample group was similar to that of control group. sample group was increased TNF-α and TGF-β production compared with control group. These results suggested that PMT had inhibitive effect on the brain damage by inhibited lactate dehydrogenase activity, IL-1β and TNF-α production, but accelerated TGF-β production. The present author thought that PMT had an anti-ischemic effect through the improvement of cerebral hemodynamics and inhibitive effect on the brain damage.
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目次
ABSTRACT = i
Ⅰ. 서론 = 1
Ⅱ. 재료 및 방법 = 3
1. 재료 = 3
1) 동물 = 3
2) 약재 = 3
2. 방법 = 3
1) 검액의 조제 = 3
2) 정상 흰쥐의 뇌혈류역학 변화 관찰 = 4
3) 정상 흰쥐의 뇌혈류역학 변화에 미치는 기전 관찰 = 6
4) 신경 세포내 lactate dehydrogenase 활성도 측정 = 6
5) 뇌허혈 병태 모델의 뇌혈류역학 변동에 미치는 개선 효과 관찰 = 7
6) 뇌허혈 병태 모델의 혈청내 사이토카인 생성능 측정 = 7
3. 통계처리 = 10
Ⅲ. 실험성적 = 11
1. PMT가 정상 흰쥐의 뇌혈류역학에 미치는 효과 = 11
2. PMT 투여로 변화된 정상 흰쥐의 뇌혈류역학 작용기전 관찰 = 13
3. PMT가 신경 세포의 LDH 활성도에 미치는 효과 = 19
4. PMT가 뇌허혈 병태 모델의 생리학적 변수에 미치는 영향 = 20
5. PMT가 뇌허혈 병태 모델의 뇌혈류역학 변동에 미치는 개선 효과 = 21
6. PMT가 뇌허혈 병태 모델의 혈청에서 생산되는 사이토카인 양에 미치는 효과 = 25
Ⅳ. 고찰 = 28
Ⅴ. 결론 = 35
참고문헌 = 37