六君子湯이 腦虛血 抑制效果에 미치는 實驗的 硏究
(The) Experimental study of Yukgunja-Tang aginst inhibitive effects on the brain ischemia
초록/요약
This experimental study was designed to investigate the effects of Yukgunja-Tang(YGJT) on the change of cerebral hemodynamics〔regional cerebral blood flow(rCBF), pial arterial diameter(PAD) and mean arterial blood pressure(MABP)〕in normal and cerebral cerebral ischemic rats, and further to determine the mechanism of action of YGJT. And, this Study was designed to investigate whether YGJT inhibit lactate dehydrogenase(LDH) activity in neuronal cells and cytokines production in serum of cerebral ischemic rats. The results in normal rats were as follows ; 1. YGJT significantly increased rCBF and PAD in a dose-dependent, and YGJT increased MABP compared with normal MABP(100 %). This results were suggested that YGJT significantly increased rCBF by dilating PAD. 2. The YGJT-induced increase in rCBF was significnatly accelerated by pretreatment with indomethacin(IDN, 1 ㎎/㎏, i.p.), an inhibitor of cyclooxygenase but was significnatly inhibited by methylene blue(MTB, 10 ㎍/㎏, i.p.), an inhibitor of guanylate cyclase. 3. The YGJT-induced increase in PAD and MABP were accelerated by pretreatment with IDN but was significnatly inhibited by MTB. This results suggested that the mechanism of YGJT is mediated by guanylate cyclase. 4. YGJT inhibited significantly LDH activity in neuronal cells. This results were suggested that YGJT prevented the neuronal death. The results in cerebral ischemic rats were as follows ; 1. Both rCBF and PAD were significantly and stably increased by YGJT(10 ㎎/㎏, i.p.) during the period of cerebral reperfusion, which contrasted with the findings of rapid and marked increase in control group. 2. In cytokine production of serum by drawing from femoral arterial blood after middle cerebral arterial occlusion(MCAO) 1 hr, IL-1β and TGF-β production of sample group were similar to that of control group, but sample group was decreased TNF-α production compared with control group, and was significantly increased IL-10 production in compared with control group. 3. In cytokine production of serum by drawing from femoral arterial blood after reperfusion 1 hr, sample group was significantly decreased IL-1β and TNF-α production compared with control group, but TGF-β production of sample group was similar to that of control group, and sample group was significantly increased IL-10 production compared with control group. 4. In cytokine production of serum by drawing from femoral arterial blood after reperfusion 4 hrs, sample group was significantly decreased IL-1β production compared with control group, and sample group was decreased TNF-α production in compared with control group, but TGF-β production of sample group was similar to that of control group, and sample group was increased IL-10 production compared with control group. This results were suggested that YGJT has inhibitive effect on the brain damage by inhibited LDH activity, IL-1β production and TNF-α production, but accelerated IL-10 production. I thought that YGJT should have an anti-ischemic effect through the improvement of cerebral hemodynamics and inhibitive effect on the brain damage.
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目次
ABSTRACT = i
Ⅰ. 緖論 = 1
Ⅱ. 材料 및 方法 = 3
1. 재료 = 3
1) 동물 = 3
2) 약재 = 3
2. 방법 = 3
1) 검액의 조제 = 3
2) 정상동물의 뇌혈류역학 변화 관찰 = 4
3) 정상동물의 뇌혈류역학 변화에 미치는 기전 관찰 = 6
4) 신경 세포내 lactate dehydrogenase 활성도 측정 = 6
5) 뇌허혈 흰쥐의 뇌혈류역학 변동에 미치는 개선 효과 관찰 = 7
6) 뇌허혈 동물의 혈청내 cytokines 생성능 측정 = 8
3. 통계처리 = 10
Ⅲ. 實驗成績 = 11
1. YGJT가 정상 흰쥐의 뇌혈류역학에 미치는 효과 = 11
2. Indomethacin 전처치 후 YGJT 투여로 변화된 정상 흰쥐의뇌혈류역학에 미치는 효과 = 14
3. Methylene blue 전처치 후 YGJT 투여로 변화된 정상 흰쥐의 뇌혈류역학에 미치는 효과 = 17
4. YGJT가 신경 세포의 LDH 활성도에 미치는 효과 = 20
5. YGJT가 뇌허혈 병태모델의 생리학적 변수에 미치는 영향 = 21
6. YGJT가 뇌허혈 병태 모델의 뇌혈류역학 변동에 미치는 개선 효과 = 22
7. YGJT가 뇌허혈 병태 모델의 혈청에서 생산되는 사이토카인 양에 미치는 효과 = 26
Ⅳ. 考察 = 30
Ⅴ. 結論 = 37
參考文獻 = 39