滋潤湯이 腦血流變化에 미치는 影響
(The) Effects of Jayun-Tang on the changes of cerebral flow
초록/요약
This study was designed to investigate the effects of Jayun-Tang extract(JYT) on the change of cerebral hemodynamics〔regional cerebral blood flow(rCBF), pial arterial diameter(PAD) and mean arterial blood pressure(MABP)〕in normal and cerebral ischemic rats, and to determine the mechanisms of action of JYT. So, we planned to investigate whether JYT inhibit lactate dehydrogenase activity in neuronal cells and cytokines production in serum of cerebral ischemic rats. The results were as follows ; 1. JYT significantly increased rCBF and PAD in a dose-dependent manner, but MABP was not changed by injecting JYT. These results suggested that JYT significantly increased rCBF by dilating PAD. 2. The JYT-induced increase in rCBF was significantly inhibited from pretreatment with indomethacin(1 ㎎/㎏, i.p.), an inhibitor of cyclooxygenase and methylene blue(10 ㎍/㎏, i.p.), an inhibitor of guanylate cyclase. 3. The JYT-induced dilation in PAD was significantly inhibited from pretreatment with indomethacin, but was increased by pretreatment with methylene blue. 4. The JYT-induced increase in MABP was reduced by pretreatment with indomethacin and methylene blue. These results suggest that the mechanism of action of JYT was mediated by cyclooxygenase. 5. JYT significantly inhibited lactate dehydrogenase activity in neuronal cells. These results suggest that JYT prevented the neuronal death. 6. Both rCBF and PAD were significantly and stably increased by JYT(10 ㎎/㎏, i.p.) during the period of cerebral reperfusion, which contrasted with the findings of rapid and marked increase in control group. 7. In cytokine production in the serum drawn from femoral artery 1 hr after middle cerebral artery occlusion, sample group showed significantly decreased production of IL-1β and TNF-α as well as increased production of IL-10 and TGF-β compared with control group. 8. In cytokine production in the serum drawn from femoral artery 1 hr after reperfusion, sample group showed significantly decreased production of IL-1β and TNF-α as well as significantly increased production of IL-10 and TGF-β compared with control group. These results suggest that JYT mediated by cyclooxygenase had inhibitive effect on the brain damage by inhibiting lactate dehydrogenase activity, IL-1β and TNF-α production, and by accelerating IL-10 and TGF-β production. The present author thought that JYT had an anti-ischemic effects through the improvement of cerebral hemodynamics and inhibitive effects on the brain damage.
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目次
ABSTRACT = ⅰ
Ⅰ. 緖論 = 1
Ⅱ. 材料 및 方法 = 3
1. 재료 = 3
1) 동물 = 3
2) 약재 = 3
2. 방법 = 4
1) 검액의 제조 = 4
2) 정상 흰쥐의 뇌혈류역학 변동 관찰 = 4
(1) 국소 뇌혈류량 측정 = 4
(2) 폐쇄 두개창 설치 = 4
(3) 뇌연막 동맥 직경 측정 = 5
(4) 평균 혈압 측정 = 5
3) 정상 흰쥐의 뇌혈류역학 변동에 미치는 기전 관찰 = 6
4) 뇌허혈 흰쥐의 뇌혈류역학 변동에 미치는 개선 효과 관찰 = 6
(1) 뇌허혈 병태모델 유발 = 6
(2) 뇌허혈 흰쥐의 rCBF 측정 = 7
(3) 뇌허혈 흰쥐의 PAD 측정 = 7
5) 생쥐의 신경 세포내 lactate dehydrogenase(이하 LDH) 활성도 측정 = 7
6) 뇌허혈 흰쥐의 혈청내 사이토카인 생성능 측정 = 8
(1) 채혈 및 혈청분리 = 8
(2) Interleukin(IL)-1β 생성능 측정 = 8
(3) Tumor necrosis factor(TNF)-α 생성능 측정 = 9
(4) IL-10 생성능 측정 = 9
(5) Transforming growth factor(TGF)-β 생성능 측정 = 10
3. 통계처리 = 10
Ⅲ. 實驗成績 = 11
1. JYT가 정상 흰쥐의 뇌혈류역학에 미치는 효과 = 11
2. 정상 흰쥐에서의 JYT에 의한 뇌혈류역학 변동기전 관찰 = 13
1) 정상 흰쥐에서의 JYT에 의한 rCBF 변동기전 관찰 = 13
2) 정상 흰쥐에서의 JYT에 의한 PAD 변동기전 관찰 =15
3) 정상 흰쥐에서의 JYT에 의한 MABP 변동기전 관찰 = 18
3. JYT가 뇌허혈 흰쥐의 생리학적 변수에 미치는 영향 = 19
4. JYT가 뇌허혈 흰쥐의 뇌혈류역학 변동에 미치는 개선 효과 = 20
1) JYT가 rCBF 변동에 미치는 개선 효과 =20
2) JYT가 PAD 변동에 미치는 개선 효과 = 22
5. JYT가 생쥐의 신경 세포 LDH 활성도에 미치는 효과 = 24
6. JYT가 뇌허혈 흰쥐 혈청의 사이토카인 생성능에 미치는 효과 = 26
1) MCA 폐색 1 시간 후 사이토카인 생성능에 미치는 효과 = 26
2) 재관류 1 시간 후 사이토카인 생성능에 미치는 효과 = 28
Ⅳ. 考察 = 30
Ⅴ. 結論 =36
參考文獻 = 38