二陳湯加味方이 肥滿에 미치는 實驗的 硏究 : 지질대사, 혈압, 뇌혈류량을 중심으로
(The) Experimental study of EjinTang-Gamybang on the obesity in Rats : Focusing on lipid-metabolism, blood pressure, cerebral blood flow
초록/요약
This experimental Study was designed to investigate the effects of EjinTang-Gamybang(ETG) on the change of weight and serum total cholesterol, HDL-cholesterol, LDL-cholesterol, triglyceride, free fatty acid, total lipid, phospholipid level in obese rat induced by high fat diet. Experimental group were as follows ; normal group were fed normal diet and administered DDW 1.0 ㎖ to rat during 7 weeks, control group were fed high fat diet and administered DDW 1.0 ㎖ during 7 weeks, sample A were fed high fat diet and administered ETG 500 ㎎/㎏ 1.0 ㎖ during 7 weeks, sample B were fed high fat diet and administered ETG 700 ㎎/㎏ 1.0 ㎖ during 7 weeks. The results were as follows ; 1. Sample A and Sample B significantly decreased body weight 4 weeks and 7 weeks in comparison with control group. 2. Sample A and Sample B significantly decreased serum LDL-cholesterol level, serum free fatty acid level, serum total lipid level, serum phospholipid level and serum leptin level in comparison with control group. 3. Sample B significantly decreased serum total cholesterol level and serum triglyceride level in comparison with control group. 4. Sample B significantly increased serum HDL-cholesterol level in comparison with control group. According to above results, the author suggested that ETG was able to be used for the herbal medication of obesity. And this Study was designed to investigate the effects of ETG on complication coming from obese rats. The author observed change of regional cerebral blood flow(rCBF), mean arterial blood pressure(MABP), heart rate(HR), cardiac muscle contractile force(CMF) in obese rats, and further improvement of rCBF in cerebral ischemic obese rats. The results were as follows ; 1. ETG significantly increased rCBF, and increased CMF in a dose-dependent. 2. ETG significantly decreased MABP in a dose-dependent. 3. rCBF was significantly and stably increased by ETG(10 ㎎/㎏, i.p.) during the period of cerebral reperfusion, which contrasted with the findings of rapid and marked increase in control group. This results were suggested that ETG significantly increased rCBF by dilating arterial diameter and activating serum leptin level. So that, the present author thought that ETG had an effects of obesity and complication coming from obesity(ischemic cerebral and cardiac disease).
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目次
ABSTRACT = 1
Ⅰ. 서론 = 1
Ⅱ. 재료 및 방법 = 3
1. 재료
1) 동물 = 3
2) 약재 = 3
2. 방법
1) 검액의 조제 = 4
2) 비만유도 및 실험군분류 = 4
3) 체중 측정 = 5
4) 채혈 및 혈청 지질 함량 측정 = 5
(1) Total cholesterol 함량 측정 = 5
(2) HDL(high density lipoprotein)-cholesterol 함량 측정 = 6
(3) LDL(low density lipoprotein)-cholesterol 함량 측정 = 6
(4) Triglyceride 함량 측정 = 6
(5) Free fatty acid 함량 측정 = 6
(6) Total lipid 함량 측정 = 6
(7) Phospholipid 함량 측정 = 7
(8) Leptin 함량 측정 = 7
5) 비만 흰쥐의 혈액 순환 변화 관찰 = 7
(1) 국소 뇌혈류량 변화 측정 = 7
(2) 평균 혈압, 심근 수축력 및 심박동수 변화 측정 = 7
(3) 뇌허혈 병태 비만 흰쥐의 뇌혈류량 변동에 미치는 개선 효과 관찰 = 8
3. 통계처리 = 9
Ⅲ. 실험성적 = 10
1. 체중 변화에 미치는 영향 = 10
2. 혈청중 total cholesterol, HDL-cholesterol, LDL-cholesterol 함량에 미치는 영향 = 12
3. 혈청중 triglyceride와 free fatty acid 함량에 미치는 영향 = 14
4. 혈청중 total lipid와 phospholipid 함량에 미치는 영향 = 16
5. 혈청중 leptin 함량에 미치는 영향 = 18
6. 국소뇌혈류량 및 평균혈압에 미치는 영향 = 19
7. 심박동수 및 심근수축력에 미치는 영향 = 21
8. 뇌허혈시 뇌혈류 변동 개선에 미치는 영향 = 23
Ⅳ. 고찰 = 25
Ⅴ. 結論 = 34
참고문헌 = 36