黃芩의 항산화 효과
Anti-oxidative effects of scutellariae radix on toxic agent induced kidney cell injury
초록/요약
This study was carried out to determine if Scutellariae Radix have protective effect against cell injury induced by various toxic agents in rat kidney slices. Water(SWe) and methanol(SMe) extracts were prepared for this experiment. Cell injury was estimated by measuring lactate dehydrogenase(LDH). Lipid peroxidation was examined by measuring malondialdehyde. SMe prevented the LDH release by CCl4, menadione, tert-butyl hydroperoxide and mercury treatment in vitro in kidney slices, but SWe prevented the LDH release by CCl4 and mercury. SMe also prevented reduction in GSH by CCl4 and lipid peroxidation induced by mercury. Thus, SMe may have more powerful efficacy on anti-oxidative effects when compared with SWe. And further studies have to be followed concerned with preparation of RR and its change of effects.
more목차
目次 = i
ABSTRACT = ⅰ
Ⅰ. 서론 = 1
Ⅱ. 재료 및 방법 = 3
1. 재료 = 3
1) 동물 = 3
2) 약재 = 3
2. 방법 = 3
1) 黃芩 추출물의 調製 = 3
2) 신장 조직 절편의 제작 = 4
3) 신장 조직에 대한 독성 약물의 처리 = 4
4) LDH 유출 측정 = 4
5) 지질의 과산화 측정 = 5
6) 환원성 glutathione(GSH)의 함량 측정 = 5
7) 통계처리 = 5
Ⅲ. 실험성적 = 7
1. CCl_(4)에 의한 LDH 유출과 이에 대한 SWe와 SMe 의 영향 = 7
2. GSH 함량에 대한 SWe와 SMe 의 영향 = 9
3. Menadione에 의한 세포 손상에 대한 SWe 및 SMe 의 영향 = 11
4. tBHP에 의한 세포 손상에 대한 SWe 및 SMe 의 영향 = 13
5. 수은에 의한 세포 손상에 대한 SWe 및 SMe 의 영향 = 15
Ⅳ. 고찰 = 18
Ⅴ. 결론 = 23
참고문헌 = 24