天麻半夏湯이 腦虛血로 인한 腦血流力學的 變動에 미치는 影響
Effect of Cheonmabanhwa-Tang on the changes in cerebral hemodynamics in cerebral ischemia of rats
초록/요약
Cheonmabanhwa-Tang (CBT) has been used in the Oriental Medicine for many centuries as a therapeutic agent for dizziness due to Poong-Dam. This study was designed to investigate whether CBT has a cytotoxicity in vitro, and affects the cerebral hemodynamics〔regional cerebral blood flow (rCBF), pial arterial diameter (PAD), and mean arterial blood pressure (MABP)〕in normal and cerebral ischemic rats, and further to determine the mechanism of action of CBT The changes of rCBF were determinated by laser-Doppler flowmetry (LDF), and the change of PAD was determinated by video-microscopy. The results in normal rats were as follows: CBT had no cytotoxicity in neuronal cells. CBT significantly increased rCBF, MABP and PAD in a dose-dependent manner, respectively. The CBT-induced increase in rCBF was significnatly inhibited by pretreatment with indomethacin (IDN, 1 ㎎/㎏, i.p.), an inhibitor of cyclooxygenase, or methylene blue (MTB, 10 ㎍/㎏, i.p.), an inhibitor of guanylate cyclase. The CBT-induced increase in PAD was also significantly inhibited by pretreatment with IDN or MTB. The CBT-induced increase in MABP was also significantly inhibited by pretreatment with IDN or MTB. The results in cerebral ischemic rats were as follows: Both rCBF and PAD were significantly and stably increased by CBT (10 ㎎/㎏, i.p.) during the period of cerebral reperfusion, which contrasted with the findings of rapid and marked increase in control group. Pretreatment with IDN significantly but unstably increased the CBT-induced increases in PAD as well as rCBF during the period of cerebral reperfusion. Pretreatment with MTB significantly but unstably increased the CBT-induced increases in PAD as well as rCBF during 150 minutes of cerebral reperfusion, but decreased stably the CBT-induced increases in rCBF and PAD after 180 minutes of cerebral reperfusion. In conclusion, it is suggested that CBT causes a diverse effect on cerebral hemodynamics via mediation of cyclooxygenase and guanylate cyclase, and thereby has an anti-ischemic action.
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ABSTRACT = i
Ⅰ. 서론 = 1
Ⅱ. 재료 및 방법 = 3
1. 재료 = 3
1) 동물 = 3
2) 약재 = 3
2. 방법 = 4
1) 검액의 조제 = 4
2) 신경세포 세포독성 측정 = 4
3) 정상동물의 뇌혈류역학 변동 및 작용기전 관찰 = 5
4) 뇌허혈 동물의 뇌혈류역학 변동 개선 및 작용기전 관찰 = 7
3. 통계처리 = 8
Ⅲ. 실험성적 = 9
1. CBT가 신경세포에 미치는 세포독성 효과 = 9
2. CBT가 정상흰쥐의 뇌혈류역학에 미치는 효과 = 10
3. Indomethacin 전처치 후 CBT 투여로 변화된 정상흰쥐의 뇌혈류역학에 미치는 효과 = 12
4. Methylene blue 전처치 후 CBT 투여로 변화된 정상흰쥐의 뇌혈류역학에 미치는 효과 = 16
5. CBT가 뇌허혈 병태 모델의 생리학적 변수에 미치는 영향 = 20
6. CBT가 뇌허혈 병태 모델의 뇌혈류역학 변동에 미치는 개선 효과 = 21
7. Indomethacin 전처치 후 CBT 투여로 변동 개선된 뇌허혈 병태 모델의 뇌혈류역학에 미치는 효과 = 25
8. Methylene blue 전처치 후 CBT 투여로 변동 개선된 뇌허혈 병태 모델에 미치는 효과 = 28
Ⅳ. 고찰 = 31
Ⅴ. 결론 = 37
참고문헌 = 39