六味地黃湯이 Alloxan으로 誘發된 생쥐의 糖尿病性 腎症에 미치는 影響
(The) Effect of Yookmijihwang-tang on mouse with diabetes nephropathy induced by alloxan
초록/요약
To investigate recovery effects of Yookmijihwang-tang, which have been used clinically in diabetes therapy, on kidney failure of a diabetes-induced mouse by alloxan administration, body and kidney weight changes of mice, BUN, creatinine, glucose, total protein and ALP activity in serum, urinalysis, excretion volume of Na+, K+ in urine were measured. The results were like below: 1. Increase of the mouse body and kidney weight and a ratio of the kidney/body weight of Yookmijihwang-tang treated group showed similar results with that of control group. 2. The BUN, creatinine, ALP activity levels in serum of Yookmijihwang-tang treated group were similar with that of control group. 3. The glucose in serum of Yookmijihwang-tang treated group were improved that of control group 4. The result of the urinalysis in the group of Yookmijihwang-tang were almost same with that of the control group. From the above results, it has been known that Yookmijihwang-tang partially improved the function of the kidney.
more목차
目次
Ⅰ. 서론 = 1
Ⅱ. 실험재료 및 방법 = 3
1. 동물 및 재료 = 3
1) 동물 = 3
2) 재료 = 3
2. 방법 = 3
1) 검액의 조제 = 3
2) 당뇨병성 신증의 유발 = 4
3) 실험동물의 분류 및 약물 투여 = 4
3. 증상관찰 = 4
4. 혈액 중 각종성분 측정 = 5
1) 혈액의 채취 및 혈장분리 = 5
2) 혈청 중 glucose, BUN/Creatinine, ALP, Protein 함량측정 = 5
5. 뇨중 각종 성분 및 뇨량 측정 = 5
1) 뇨량 측정 = 5
2) Screening by Urinary Test Strip = 5
3) 뇨중 Sodium(Na+), Potassiuim(K+)량 측정 = 5
6. 신장의 적출 및 신장조직내 Protein, malondialdehyde(MDA)량 측정 = 6
1) 신장의 적출 = 6
2) 신장 조직내 protein량 측정 = 6
3) 신장조직 malondialdehyde(MDA)량 측정 = 6
7. 통계처리 = 7
Ⅲ. 실험결과 = 8
1. 당뇨유도확인시험 = 8
2. 당뇨유발에 의한 신장기능 저하 = 8
3. 10일간 연속투여에 따른 신장기능회복효과 = 9
1) 신장/체중 무게비의 변화 = 9
2) 혈액 생화학적 검사 = 10
3) 뇨검사 = 13
4) 뇨 생화학적 검사 = 15
5) 과산화지질 생성량 = 17
Ⅳ. 고찰 = 18
Ⅴ. 결론 = 27
참고문헌 = 29
ABSTRACT = 32