韓藥複合處方의 藥鍼및 口腔投與가 Streptozotocin에 의한 흰쥐의 糖尿病性 腎症에 미치는 영향
Beneficial effect of the combination of oral administration and herbal-acupuncture stimulation with several herb-combined prescription on streptozotocin-induced diabetic nephropathy rats
초록/요약
The present study was carried out to investigate the preventive effect of Several Herb-Combined Prescription(SHP) on streptozotocin(STZ)-induced diabetic nephropathy. SHP was given to rats with the combination of oral administration and herbal-acupuncture stimulation. The experimental animals were divided into 3 groups : normal group of rats, control group of STZ-induced diabetic rats, sample group with SHP treatment. Experimental diabetes was induced by the injection of STZ(60㎎/㎏) to the rat via the peritoneum. The effect of SHP on STZ-induced diabetic nephropathy was observed by measuring the serum level of creatinine and BUN. Urine secretion of albumin for 24 hours and urine level of glucose measures too. Anti-oxidative stress of SHP administration in living body was estimated by measuring lipid peroxide and GSH content in cortex of kidneys. STZ induced increase of serum creatinine, BUN and albumin secretion were lowered by SHP treatment. In conclusion, the SHP treatment showed protective effect on rat diabetic nephropathy model, and action mechanism of the effect was thought to be concerned with anti-oxidative stress.
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目次 = i
ABSTRACT = ⅲ
Ⅰ. 緖論 = 1
Ⅱ. 實驗材料 및 方法 = 2
1. 材料 = 2
1) 동물 = 2
2) 처방 및 구성 약재 = 2
3) 시약 및 기기 = 3
2. 方法 = 3
1) 처방의 추출 = 3
2) 藥鍼液의 제조 = 3
3) 藥鍼 穴位 선정 및 藥鍼 시술 방법 = 4
4) 당뇨병성 신증의 유도 및 검액 투여 = 5
5) 혈액 채취, 혈청 분리, 혈청 중 creatinine 및 BUN 함량 측정 = 5
6) 뇨의 수집, albumin 배설량 및 뇨 중 glucose 함량 측정 = 5
7) 신장 피질의 지질 과산화 정도 측정 = 6
8) 신장 피질 내 GSH 함량 측정 = 6
3. 統計 處理 = 7
Ⅲ. 成績 = 8
1. 혈청 중 creatinine 함량 = 8
2. 혈청 중 BUN 함량 = 9
3. 24시간 동안의 albumin 배설량 = 10
4. 뇨 중 glucose 함량 = 11
5. 신장 피질의 과산화 지질 함량 = 12
6. 신장 피질 내 GSH 함량 = 13
Ⅳ. 考察 = 14
Ⅴ. 結論 = 19
參考文獻 = 20