蔘기地黃湯이 Streptozotocin으로 유발된 흰쥐의 糖尿病性腎症에 미치는 영향
Effect of Samgijiwhang-Tang on diabetic nephropathy rats induced streptozotocin
- 주제(키워드) 삼기지황탕 , Streptozotocin , 당뇨병성신증
- 발행기관 東新大學校 大學院
- 발행년도 2004
- 학위명 석사
- 학과 한의학과
초록/요약
The present study was carried out to investigate the preventive effect of Samgijiwhang-Tang(SJT) on streptozotocin(STZ)-induced diabetic nephropathy. SJT was given to rats with oral administration. The experimental animals were divided into normal group of rats, control group of STZ-induced diabetic rats, and sample group with SJT administration. Rehmanniae Radix Preparat is combined in the original prescription of SJT, but in this experiment, Rehmanniae Radix was combined instead of Rehmanniae Radix Preparat to compare the effect of anti diabetic nephropathy. Experimental diabetic nephropathy was induced by the injection of STZ(60㎎/㎏) to the rat via the peritoneum. The effect of SJT on STZ-induced diabetic nephropathy was observed by measuring the serum level of creatinine and BUN. Urine secretion of albumin for 24 hours and urine level of glucose measures too. Anti-oxidative stress of SJT administration in living body was estimated by measuring lipid peroxide and GSH content in cortex of kidneys. STZ induced increase of serum creatinine, urine glucose and renal cortical lipid peroxidation were lowered by Rehmanniae Radix Preparat combined SJT administration. In conclusion, the SJT treatment showed protective effect on rat diabetic nephropathy model. Especially effect of Rehmanniae Radix combined SJT administration is better than effect of Rehmanniae Radix Preparat combined SJT administration mostly. And action mechanism of the effect was thought to be concerned with anti-oxidative stress.
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目次 = i
ABSTRACT = ⅲ
Ⅰ. 序論 = 1
Ⅱ. 材料 및 方法 = 2
1. 材料 = 2
1) 동물 = 2
2) 처방 및 구성 약재 = 2
3) 시약 및 기기 = 3
2. 方法 = 4
1) 검액의 추출 = 4
2) 糖尿病性 腎症의 유도 및 검액 투여 = 4
3) 혈액 채취, 혈청 분리, 혈청 중 creatinine 및 BUN 함량 측정 = 5
4) 뇨 중 albumin 배설량 및 glucose 함량 측정 = 5
5) 신장 피질의 지질 과산화 정도 측정 = 5
6) 신장 피질 내 GSH 함량 측정 = 6
3. 統計 處理= 6
Ⅲ. 成績 = 7
1. 혈청 중 creatinine 함량 = 7
2. 혈청 중 BUN 함량 = 8
3. 24시간 동안의 albumin 배설량 = 9
4. 뇨 중 glucose 함량 = 10
5. 신장 피질의 과산화 지질 함량 = 11
6. 신장 피질 내 GSH 함량 = 12
Ⅳ. 考察 = 13
Ⅴ. 結論 = 22
參考文獻 = 23