六味地黃湯이 局所腦虛血로 유발된 記憶力損傷白鼠에 미치는 影響
(The) Effects of Yukmijihwang-Tang(UJT) on dementia induced by focal brain ischemic injury in rats
초록/요약
Objective : Yukmijihwang-Tang(UJT) has been used for Dementia derived by deficiency of Kidney-Yin in the oriental medicine. This study was planned to examine the effects of UJT on the memory loss induced by focal brain ischemic injury in the rats. Methods : Experimental groups were divided into 4 groups ; Normal group, Control group, UJT1 group and UJT2 group. Control group were no treated after focal brain ischemic injury. UJT1 group were administered UJT 0.3 ㎖/㎏ to focal brain ischemic injuried rats for 21 days, UJT2 group were administered UJT 1.2 ㎖/㎏ to focal brain ischemic injuried rats for 21 days. The present author observed the number of errors on the eight-arm radial maze task, the rate of correct choice on the eight-arm radial maze task, the values of density of Cresyl violet-stained sections in the hippocampal CA1 and the values of density of Acetlycholine Esterase (AchE)stained nuclei in the hippocampal CA1. The results were as follows : The number of errors in the Eigth-arm radial maze task was significantly decreased in UJT1 group on 1, 2, 3, 5, 6days, And it was significantly decreased in UJT2 group on 1-6days compared with control group. The rate of correct choice in the eight-arm radial maze task was significantly increased in UJT1, UJT2 group compared with control group. The values of density of Cresyl violet-stained sections in the hippocampal CA1 were significantly increased in UJT1, UJT2 group compared with control group. The values of density of AchE in the hippocampal CA1 were increased in UJT1, UJT2 group compared with control group, but the values were not significant. Conclusions : The present author thought that Yukmijihwang-Tang could be used for curing dementia induced by focal brain ischemic injury.
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目次
ABSTRACT = ⅰ
Ⅰ. 緖論 = 1
Ⅱ. 材料 및 方法 = 3
1. 실험동물 = 3
2. 군의 분리 = 3
3. 검액조제 = 3
4. 실험절차 = 4
5. Occlusion에 의한 focal brain ischemic injury 유발 = 5
6. Eight-arm radial maze task = 5
7. Immunohistochemistry = 7
1) Cresyl violet 염색 = 7
2) Acetylcholinesterase(AchE) 염색 = 8
8. 통계처리 = 8
Ⅲ. 成績 = 9
1. 방사형 미로 학습에서 誤行路 선택수 = 9
2. 방사형 미로 학습에서 正行路 선택율 = 11
3. Cresyl violet을 이용한 신경세포 손상 방어효과 = 13
4. AchE 발현에 미치는 영향 = 16
Ⅳ. 考察 = 19
Ⅴ. 結論 = 25
參考文獻 = 26