蔘기地黃湯加淫羊藿이 Streptozotocin으로 유발된 흰쥐의 糖尿病性 腎症에 미치는 영향
Effect of epimedii herba combined Samgijiwhang-Tang on diabetic nephropathy rats induced by streptozotocin
- 주제(키워드) 삼기지황탕가음양곽 , Streptozotocin , 당뇨병 , 신증
- 발행기관 東新大學校 大學院
- 발행년도 2004
- 학위명 석사
- 학과 한의학과
초록/요약
The present study was carried out to investigate the preventive effect of Epimedii Herba combined Samgijiwhang-Tang(SJTE) on streptozotocin(STZ)-induced diabetic nephropathy. SJTE was given to rats with oral administration. The experimental animals were divided into normal group of rats, control group of STZ-induced diabetic rats, and sample group with SJTE administration. Experimental diabetic nephropathy was induced by the injection of STZ(60㎎/㎏) to the rat via the peritoneum. The effect of SJTE on STZ-induced diabetic nephropathy was observed by measuring the serum level of insulin, glucose, creatinine and BUN. Urine secretion of albumin for 24 hours and urine level of glucose measures too. Anti-oxidative stress of SJTE administration in living body was estimated by measuring lipid peroxide in cortex of kidneys. STZ induced at the rats increasing of serum glucose, creatinine, urine albumin secretion and renal cortical lipid peroxidation and SJTE administration lowered that. In conclusion, the SJTE treatment showed protective effect on rat diabetic nephropathy model, and action mechanism of the effect was thought to be concerned with internal glucose metabolism.
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ABSTRACT = ⅲ
Ⅰ. 서론 = 1
Ⅱ. 재료 및 방법 = 2
1. 재료 = 2
1) 동물 = 2
2) 처방 및 구성 약재 = 2
3) 시약 및 기기 = 3
2. 방법 = 3
1) 검액의 추출 = 3
2) 당뇨병성 신증의 유도 및 검액 투여 = 3
3) 혈청 중 인슐린, glucose, creatinine 및 BUN 함량 측정 = 4
4) 뇨의 수집, albumin 배설량 및 뇨중 glucose 함량 측정 = 4
5) 신장 피질의 지질 과산화 정도 측정 = 4
3. 통계처리 = 5
Ⅲ. 성적 = 6
1. 혈청 중 인슐린 함량 변화 = 6
2. 혈청 중 glucose 함량 변화 = 7
3. 혈청 중 creatinine 함량 변화 = 8
4. 혈청 중 BUN 함량 변화 = 9
5. 24시간 뇨중 albumin 배설량 변화 = 10
6. 뇨 중 glucose 함량 변화 = 11
7. 신장 피질의 과산화 지질 함량 변화 = 12
Ⅳ. 고찰 = 13
Ⅴ. 결론 = 18
參考文獻 = 19