韓藥複合處方 藥鍼 및 經口投與가 Streptozotocin에 의한 흰쥐의 糖尿病과 抗酸化能에 미치는 영향
Beneficial Effect of the Combination of Oral Administration and Herbal-Acupuncture Stimulation with Several Herb-combind Prescription on Streptozotocin-Induced Diabetic Rats
초록/요약
The present study was carried out to investigate the preventive effect of Several Herb-combind Prescription(SHP) on Streptozotocin(STZ) -induced Diabetes mellitus. SHP was given to rats with the combination of oral administration and herbal-acupuncture stimulation. The experimental animals were divided into 3 groups normal group of rats, control group of STZ-induced diabetic rats, sample group with SHP treatment. In vitro test of SHP showed α-glucosidase inhibition, DPPH radical scavenging activity and inhibition of lipid peroxidation. Experimental diabetes was induced by the injection of STZ(60mg/kg) to the rat via the peritoneum. The effect of SHP on STZ-induced diabetes was observed by measuring the serum level of insulin, glucose, triglyceride, total cholesterol and lipid peroxides. Hepatic activities of catalase and reduced glutathione were examined and insulin granule was observed by immunohistochemical examination. STZ caused hyperglycemia and hypoinsulinemia by a selectively destroying pancreatic cell. SHP treatment protected them from the hyperglycemia and hypoinsulinernia. STZ induced increase of serum triglyceride lowered by SHP treatment. And by SHP treatment, pancrease showed a big area with positive immuno-reactivity for presence of insulin with many insulin granules distributed in the β-cells in the islets of Langerhans. The SHP treatment showed protective effect on diabetic rat model, and action mechanism of the effect was thought to be concerned with anti-oxidative stress.
more목차
목차 = I
ABSTRACT = III
I. 緖論 = 1
II. 材料 및 方法 = 2
1. 재료 = 2
1) 동물 = 2
2) 처방 및 구성 약재 = 2
3) 시약 및 기기 = 3
4) 시료의 추출 = 3
5) 약침액의 제조 = 3
2. 방법 = 4
1) 혈위선정 및 약침시술 = 4
2) 당뇨병의 유도 및 시료 투여 = 5
3) SHP의 α-glucosidase 저해 활성 측정 = 5
4) DPPH 라디찰 소거능 측정 = 5
5) 지질 과산화 억제 효과 측정 = 6
6) 실험동물의 혈청 조성 변화 측정 = 7
7) 생체 내에서의 항산화 효과 측정 = 7
8) 면역조직화학 염색 = 8
3. 통계처리 = 8
III. 成籍 = 10
1. In vitro에서 SHP의 α-glucosidase 저해 활성 = 10
2. DPPH에 의한 항산화능 = 12
3. 지질 과산화 억제 효과 = 14
4. 혈청 중 insulin 함량 변화 = 16
5. 혈청 중 glucose 함량 변화 = 18
6. 혈청 중 triglyceride 함량 변화 = 20
7. 혈청 중 total cholesterol 함량 변화 = 22
8. 혈청 중 지질 과산화물 함량 변화 = 24
9. 간 조직 내 catalase 활성 변화 = 26
10. 간 조직 내 GSH 활성 변화 = 28
11. 췌장의 면역조직화학적 변화 = 30
IV. 考察 = 31
V. 結論 = 37
參考文獻 = 39