化痰通絡湯이 白鼠의 腦血流變化 및 虛血性 腦損傷에 미치는 影響
Effects of Hwadamtongrak-Tang on the changes of Cerebral Flow in the rats
초록/요약
This present study was performed to investigate the effect of Hwadamtongrak-Tang extract (HTT) on the regulation of cerebral hemodynamics in terms of regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP)〕in normal and cerebral ischemic rats. Also the effects of HTT on changes in local blood flow, inhibition of LDH activity in neuronal cells, and levels of cytokine production in the serum were determined in the ischemic rat model. The major findings are summarized below. 1. HTT significantly increased rCBF in a dose-dependent manner, but MABP was not changed by HTT treatment. These results suggest that HTT may increase rCBF by dilating cerebral arterial diameter. 2. HTT-induced increase in rCBF was blocked by pretreatment with cyclooxygenase inhibitor indomethacin (IDN, 1 ㎎/㎏, i.p.) and MABP was significantly increased by IDN. 3. Pretreatment of methylene blue (MTB, 10 ㎍/㎏, i.p.), an inhibitor of guanylate cyclase, significantly decreased both rCBP and MABP in HTT-treated rats. 4. HTT treatment significantly increased rCBP to a stable level during the period of cerebral reperfusion. 5. HTT significantly inhibited LDH activity in neuronal cells, suggesting a neuroprotection by HTT. 6. Serum interleukin (IL)-1β and tumor necrosis factor (TNF)-α levels were significantly decreased in the femoral artery 1 hr after middle cerebral arterial occlusion in HTT-treated rats. IL-10 levels in the serum were significantly increased by HTT treatment whereas transforming growth factor (TGF)-β levels were similar between HTT-treated and control groups. 7. Serum interleukin (IL)-1β and tumor necrosis factor (TNF)-α levels were significantly decreased in the femoral artery 1 hr after reperfusion in HTT-treated rats. Serum IL-10 levels were significantly decreased in HTT-treated rats compared with the control group, and no significant changes in (TGF)-β in the serum were observed by HTT treatment. The present data suggest that HTT may have an anti-ischemic effect via the improvement of cerebral hemodynamics and thus protect the brain from ischemic damage.
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目次
ABSTRACT = ⅰ
Ⅰ. 緖論 = 1
Ⅱ. 材料 및 方法 = 2
1. 재료 = 2
1) 동물 = 2
2) 약재 = 2
2. 방법 = 3
1) 검액의 제조 = 3
2) 정상 백서의 뇌혈류역학 변화 = 3
3) 정상 백서의 뇌혈류역학 변화에 미치는 기전 = 4
4) 뇌허혈 병태 모델의 뇌혈류역학 변동에 미치는 개선 효과 = 4
5) 생쥐의 신경세포내 LDH 활성도 측정 = 4
6) 뇌허혈 병태 모델의 혈청내 사이토카인 생성능 측정 = 5
3. 통계분석 = 8
Ⅲ. 實驗成績 = 9
1. 化痰通絡湯이 정상 백서의 뇌혈류역학에 미치는 효과 = 9
2. 化痰通絡湯 투여로 변화된 정상 백서의 뇌혈류역학 작용기전 = 11
1) 化痰通絡湯 투여로 변화된 정상 백서의 국소 뇌혈류량 작용기전 = 11
2) 化痰通絡湯 투여로 변화된 정상 백서의 평균혈압 작용기전 = 13
3. 化痰通絡湯이 뇌허혈 병태모델의 국소 뇌혈류량 변동에 미치는 개선 효과 = 15
4. 化痰通絡湯이 생쥐의 신경세포의 LDH 활성도에 미치는 효과 = 17
5. 化痰通絡湯이 뇌허혈 병태 모델의 혈청에서 생성되는 사이토카인 양에 미치는 효과 = 18
1) 중대뇌동맥 폐색 1 시간 후 생성되는 사이토카인 양에 미치는 효과 = 18
2) 재관류 1 시간 후 생성되는 사이토카인 양에 미치는 효과 = 20
Ⅳ. 考察 = 22
Ⅴ. 結論 = 28
참고문헌 = 30