大敦·少衝·少府 刺鍼이 腦血流力學에 미치는 영향
Experimental Study of Dadun(LR1)·Shaofu(HT8)·Shaochung(HT9) Reinforcement in Acupuncture on the Improvement of Cerebral Hemodynamics
초록/요약
LR1 and HT8, HT9 have been used in oriental medicine for many centries as a meridian point to treat apoplexy. And, I designed to investigate whether LR1·HT8·HT9 Reinforcement in Acupuncture affects cerebral hemodynamics〔regional cerebral blood flow(rCBF), pial arterial diameter(PAD), mean arterial blood pressure(MABP)〕in normal and cerebral ischemia rats by MCA occlusion method, and to make manifest whether LR1·HT8·HT9 Reinforcement in Acupuncture is mediated by cyclooxygenase or guanylate cyclase. The changes of rCBF and MABP were determinated by laser-doppler flowmetry(LDF), and the change of PAD was determinated by video microscope and width analyzer. The results were as follows in normal rats ; 1. LR1·HT8·HT9 Reinforcement in Acupuncture was significantly increased rCBF, PAD, but decreased MABP after withdrawing of the needle. This results suggest that LR1·HT8·HT9 Reinforcement in Acupuncture increased significantly rCBF by dilating PAD. 2. Pretreatment with indomethacin(1㎎/㎏, ⅰ.ⅴ.) was significantly inhibited LR1·HT8·HT9 Reinforcement in Acupuncture induced increase of rCBF and PAD, but increased LR1·HT8·HT9 Reinforcement in Acupuncture induced decrease of MABP after withdrawing of the needle. 3. Pretreatment with methylene blue(10㎍/㎏, ⅰ.ⅴ.) was decreased LR1·HT8·HT9 Reinforcement in Acupuncture induced increase of rCBF and MABP, but accelerated LR1·HT8·HT9 Reinforcement in Acupuncture induced decrease of PAD. This results suggest that the mechanism of LR1·HT8·HT9 Reinforcement in Acupuncture is mediated by cyclooxygenase or guanylate cyclase. And the results were as follows in cerebral ischemic rats ; 4. The changes of rCBF was increased stabilizly by treatment with LR1·HT8·HT9 Reinforcement in Acupuncture during the period of cerebral reperfusion. 5. The changes of PAD was dilated stabilizly by treatment with LR1·HT8·HT9 Reinforcement in Acupuncture during the period of cerebral reperfusion 6. Pretreatment with indomethacin was significantly increased LR1·HT8·HT9 Reinforcement in Acupuncture induced increase of rCBF and PAD during the period of cerebral reperfusion. 7. Pretreatment methylene blue was decreased LR1·HT8·HT9 Reinforcement in Acupuncture induced increase of rCBF and PAD during the period of cerebral reperfusion. This results suggest that the mechanism of LR1·HT8·HT9 Reinforcement in Acupuncture is mediated by cyclooxygenase. In conclusion, I suggest that LR1·HT8·HT9 Reinforcement in Acupuncture has an anti-ischemic effect through the improvement of crebral hemodynamics, and the mechanism is mediated by cyclooxygenase.
more목차
目次
ABSTRACT = ⅰ
Ⅰ. 緖論 = 1
Ⅱ. 材料 및 方法 = 2
1. 재료 = 2
2. 방법 = 2
1) 정상 백서의 뇌혈류역학 변화 및 작용기전 관찰 = 4
2) 뇌허혈 백서의 뇌혈류역학 변동 개선 및 작용기전 관찰 = 7
3. 통계처리 = 8
Ⅲ. 成績 = 9
1. 정상 백서의 뇌혈류역학에 미치는 효과 = 9
2. Indomethacin 전처치 후 정상 백서의 뇌혈류역학 변화에 미치는 효과 = 15
3. Methylene blue 전처치 후 정상 백서의 뇌혈류역학 변화에미치는 효과 = 21
4. 뇌허혈 백서의 뇌혈류역학 변동에 미치는 개선 효과 = 27
5. Indomethacin 전처치 후 뇌허혈 백서의 뇌혈류역학 변동개선에 미치는 효과 = 31
6. Methylene blue 전처치 후 뇌허혈 백서의 뇌혈류역학 변동개선에 미치는 효과 = 35
Ⅳ. 考察 = 39
Ⅴ. 結論 = 46
Ⅵ. 參考文獻 = 48