竹瀝과 대나무 抽出液의 混合物이 腦血流 및 血壓에 미치는 影響
Effects of Mixture of Bambusae Caulis in Liquamen and Bamboo Extract on the Cerebral Blood Flow and Blood Pressure in Rats
초록/요약
This Study was designed to investigate the effects of Mixture of Bambusae Caulis in Liquamen and Bamboo Extract on the change of regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in normal and cerebral ischemic rats, and further to determine the mechanisms of action of mixture. And, this study was designed to investigate cytokines production in serum of cerebral ischemic rats. Experimental materials were as follows ; BE-Ⅰ was Bamboo Extract (BE) extracted with 70% ethyl alcohol, BE-Ⅱ was BE extracted with distilled water at 121 ℃ for 30 min, BE-Ⅲ was BE extracted with distilled water at 98 ℃ for 3 hrs, MLC was mixture of Bambusae Caulis in Liquamen (BCL) and BE-Ⅲ mixed at the ratio of 1 to 100 (MLC100), 1 to 50 (MLC50), 1 to 20 (MLC20), 1 to 10 (MLC10), 1 to 5 (MLC5). The results were as follows ; The Changes of BE-Ⅰ on the rCBF and MABP in normal rats were not showed, BE-Ⅱ significantly decreased rCBF in a dose-dependent manner but increased MABP in a dose-dependent manner. BE-Ⅲ increased rCBF in a dose-dependent manner, MLC significantly increased rCBF in a dose-dependent manner and increased MABP in a dose-dependent manner. The MLC-induced increase in rCBF was significantly inhibited by pretreatment with indomethacin (1 ㎎/㎏, i.p.), an inhibitor of cyclooxygenase as well as methylene blue (10 ㎍/㎏, i.p.), an inhibitor of guanylate cyclase. The MLC-induced increase in MABP was significantly inhibited by pretreatment with methylene blue. rCBF was significantly and stably increased by MLC5 (1 ㎖/㎏, i.p.) during the period of cerebral reperfusion, which contrasted with the findings of rapid and marked increase in control group. In cytokines production in the serum drawn from femoral arterial 1 hr after middle cerebral artery occlusion, MLC5 significantly increased production of TGF-β and increased production of IL-10, but significantly decreased production of TNF-α compared with control group. In cytokines production in the serum drawn from femoral arterial 1 hr after reperfusion, MLC5 significantly increased production of TGF-β and IL-10, but significantly decreased production of TNF-α compared with control group. As results above ; The present author thought that BE-Ⅲ and MLC increased rCBF by dilating pial arterial diameter, effect of MLC was mediated by guanylate cyclase. And MLC5 had anti-ischemic effect by inhibiting TNF-α production, and by accelerating IL-10 and TGF-β production.
more목차
목차
ABSTRACT = ⅰ
Ⅰ. 서론 = 1
Ⅱ. 재료 및 방법 = 3
1. 재료 = 3
1) 동물 = 3
2) 시료 = 3
2. 방법 = 7
1) 정상 흰쥐의 국소 뇌혈류량 및 평균 혈압에 미치는 변화 측정 = 7
2) 뇌허혈 흰쥐의 국소 뇌혈류량 변동 개선 효과 측정 = 8
3) 뇌허혈 흰쥐의 혈청내 사이토카인 생성능 측정 = 8
3. 통계처리 = 11
Ⅲ. 실험성적 = 12
1. 대나무 추출액 Ⅰ (BE-Ⅰ)이 국소 뇌혈류량 및 평균 혈압에 미치는 효과 = 12
2. 대나무 추출액 Ⅱ (BE-Ⅱ)가 국소 뇌혈류량 및 평균 혈압에 미치는 효과 = 14
3. 대나무 추출액 Ⅲ (BE-Ⅲ)이 국소 뇌혈류량 및 평균 혈압에 미치는 효과 = 16
4. 죽력과 대나무 추출액 Ⅲ의 혼합물 (MLC)이 국소 뇌혈류량 및 평균 혈압에 미치는 효과 = 18
5. 죽력과 대나무 추출액 Ⅲ의 혼합물 (MLC)투여로 변화된 정상 흰쥐의 국소 뇌혈류량 작용기전 관찰 = 20
6. 죽력과 대나무 추출액 Ⅲ의 혼합물 (MLC)투여로 변화된 정상 흰쥐의 평균 혈압 작용기전 관찰 = 22
7. 죽력과 대나무 추출액 Ⅲ의 혼합물 (1:5 농도, MLC5)이뇌허혈 흰쥐의 국소 뇌혈류량 변동 개선에 미치는 효과 = 24
8. 죽력과 대나무 추출액 Ⅲ의 혼합물 (MLC5)이 뇌허혈 흰쥐의 혈청에서 생성되는 사이토카인 양에 미치는 효과 = 26
1) 중대뇌동맥 폐색 (MCAO) 1 시간 후 생성되는 사이토카인 양에미치는 효과 = 26
2) 재관류 1 시간 후 생성되는 사이토카인 양에 미치는 효과 = 28
Ⅳ. 고찰 = 30
Ⅴ. 결론 = 37
참고문헌 = 39