녹용 약침이 국소 뇌허혈 모델 백서에서 방사형 미로과제 학습 및 뇌신경 보호에 미치는 영향
Effect of Cervus Elaphus-Herbal Acupuncture on Focal Ischemia Induced by Inserted Intraluminal Filament in MCA of Rats
초록/요약
Objectives : Cervus Elaphus(CE) has been used to medication for ischemic stroke in the Oriental Medicine. So this study was planned to investigate the effects of CE herbal acupuncture therapy(CE-HAT) on the focal ischemia-induced by intraluminal filament insertion in the rats. Materials and methods : The focal ischemia was induced by middle cerebral artery occlusion for 2hours. The groups divided into 6 groups, normal(intactness group), control(no therapy group after ischemia-induced), CE-HAT1(Cervus Elaphus-herbal acupuncture therapy group at LR1, HT9 after ischemia-induced), CE-HAT2(Cervus Elaphus-herbal acupuncture therapy group at LU8, KI7 after ischemia-induced), CE-HAT3(Cervus Elaphus-herbal acupuncture therapy group at LR1, HT9, LU8, KI7 after ischemia-induced), CE-HAT4(Cervus Elaphus-herbal acupuncture therapy group at LR1, HT9, HT3, KI10 after ischemia-induced). CE-HAT was carried out during 3 weeks after focal ischemia-induced. Eight-arm radial maze was used for the behavioral task and neuropretective effect of CE-HAT was observed by Cresyl violet, AchE, ChAT-stain. Results : The error rate in the eight-arm radial maze task was significantly decreased in CE-HAT1, CE-HAT2, CE-HAT4 on 3days, CE-HAT4 on 4days, CE-HAT2, CE-HAT4 on 5days, CE-HAT3, CE-HAT4 on 6days. The rate of correct choice was significantly increased in CE-HAT4. The density of neurons in the hippocampal CA1 was significantly increased in CE-HAT1, CE-HAT2, CE-HAT3, CE-HAT4, compared to control group. The density of AchE in the hippocampal CA1 was significantly increase in CE-HAT4. The density of ChAT in the hippocampal CA1 was significantly increased CE-HAT1. Conclusions : These results suggest that the Cervus Elaphus-herbal acupuncture therapy could be used as a medication for controlling the stroke induced by deficiency.
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目次
ABSTRACT = ⅰ
Ⅰ. 緖論 = 1
Ⅱ. 재료 및 방법 = 2
1. 실험동물 = 2
2. 실험군의 구성 및 절차 = 2
3. 약물 제법 및 약침 시술 = 2
4. Occlusion에 의한 focal ishcemia 유발 = 3
5. Eight-arm radial maze task = 3
6. Immunohistochemistry = 4
7. 통계처리 = 6
Ⅲ. 成績 = 7
1. 방사형 미로 학습의 획득시행동안 주로 오선 횟수 = 7
2. 방사형 미로 학습의 검사시행동안 주로 정선택율 = 9
3. Cresyl violet을 이용한 신경세포 손상 방어효과 = 10
4. Acetylcholinesterase (AchE)발현 = 12
5. Choline acetyltransferase (ChAT)발현 = 14
Ⅳ. 考察 = 16
Ⅴ. 結論 = 20
參考文獻 = 21