검색 상세

木香調氣散 추출물이 뇌허혈 손상에 미치는 실험적 효과

Experimental Effects of Mokhyangjokisan Extract on the Cerebral Ischemia in Rats

  • 심익현 (Shim, Ik-Hyun, 동신대학교 대학원)

초록/요약

This study was designed to investigate the effects of Mokhyangjokisan extract (MJS) on the changes in regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in normal and cerebral ischemic rats, and further to determine the mechanism of action and cytokines production (IL-1β, TNF-α, IL-10, TGF-β) of MJS. The results in normal rats were as follows ; MJS significantly increased rCBF in a dose-dependent manner, while it did not change MABP. This result suggests that MJS significantly increased rCBF by dilating pial arterial diameter. Increase of MJS-induced rCBF was significantly inhibited by pretreatment with methylene blue (10 ㎍/㎏, i.p.), an inhibitor of guanylate cyclase, and was not changed by indomethacin (1 ㎎/㎏, i.p.), an inhibitor of cyclooxygenase. MJS-induced MABP was increased by pretreatment with indomethacin but was not changed by methylene blue. These results suggested that the action of MJS was mediated by cyclic 3',5'-guanosine monophosphate. The results in cerebral ischemic rats were as follows ; The rCBF was significantly and stably increased by MJS (10 ㎎/㎏, i.p.) during the period of cerebral reperfusion, which contrasted with the findings of rapid and marked increase in control group. In cytokine production in serum from femoral arterial blood 1 hr after middle cerebral arterial occlusion, MJS significantly decreased IL-1β and TNF-α production, and increased IL-10 production compared with control group. In cytokine production in serum from femoral arterial blood 1 hr after reperfusion, MJS significantly decreased IL-1β and TNF-α production compared with control group. IL-10 and TGF-β1 production in MJS group were significantly increased compared with control group. These results suggested that MJS significantly and stably increased rCBF by inhibiting the production IL-1β and TNF-α, and accelerating that of IL-10 and TGF-β. The result in nerve cells was as follows ; MJS significantly inhibited lactate dehydrogenase activity in vitro in a dose-dependent manner. This result suggested that MJS prevented the neuronal death.

more

목차

Ⅰ. 서론 = 1
Ⅱ. 재료 및 방법 = 3
1. 재료 = 3
1) 동물 = 3
2) 약재 = 3
2. 방법 = 4
1) 검액의 조제 = 4
2) 정상 흰쥐의 뇌혈류역학 변화 관찰 = 4
3) 정상 흰쥐의 뇌혈류역학 변화에 미치는 기전 관찰 = 5
4) 뇌허혈 유발 흰쥐의 뇌혈류역학 변동에 미치는 개선 효과 관찰 = 6
5) 뇌허혈 유발 흰쥐의 혈청내 사이토카인 양 측정 = 7
6) In vitro 상에서 신경세포내 lactate dehydrogenase 활성도 측정 = 9
3. 통계처리 = 10
Ⅲ. 성적 = 11
1. 정상 흰쥐의 국소 뇌혈류량 및 평균 혈압에 미치는 효과 = 11
2. 정상 흰쥐의 국소 뇌혈류량 작용기전 관찰 = 13
3. 정상 흰쥐의 평균 혈압 작용기전 관찰 = 15
4. 뇌허혈 유발 흰쥐의 국소 뇌혈류량 변동에 미치는 개선 효과 = 17
5. 뇌허혈 유발 흰쥐의 혈청에서 생성되는 사이토카인 양에 미치는 효과 = 19
1) 중뇌동맥 폐색 1시간 후 생성되는 사이토카인 양에 미치는 효과 = 19
2) 재관류 1시간 후 생성되는 사이토카인 양에 미치는 효과 = 21
6. 생쥐의 신경세포내 LDH 활성도에 미치는 효과 = 23
Ⅳ. 고찰 = 24
Ⅴ. 결론 = 32
참고 문헌 = 34

more
반출 Meta View 목록