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鷄血藤 추출물이 일과성 뇌허혈로 인한 뇌손상에 미치는 효과

Effects of Patholobi Caulis on the Brain Damage by Transient Cerebral Ischemia in Rats

  • 이상록 (Lee, Sang-Lok, 동신대학교 대학원)

초록/요약

The study was designed to investigate the effects of Patholobi Caulis freeze dried powder (PCF) on the changes in regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in normal and cerebral ischemic rats, and further to determine the mechanism and cytokines production (IL-1β, TNF-α, IL-10, TGF-β) of PCF. 1. The results in normal rats were as follows ; PCF significantly increased rCBF in a dose-dependent manner, and increased MABP in a dose-dependent manner. This result suggested that PCF significantly increased rCBF by increasing MABP and dilating pial arterial diameter. Increase of PCF-induced rCBF was significantly inhibited by pretreatment with methylene blue (10 ㎍/㎏, i.p.), an inhibitor of guanylate cyclase, and was inhibited by indomethacin (1 ㎎/㎏, i.p.), an inhibitor of cyclooxygenase. Increase of PCF-induced MABP was decreased by pretreatment with methylene blue, but was increased by indomethacin. These results suggested that the mechanism of action PCF was mediated by cyclic 3',5'-guanosine monophosphate. 2. The results in cerebral ischemic rats were as follows ; PCF significantly inhibited lactate dehydrogenase activity in nerve cells. This result suggested that PCF prevented the neuronal death. The rCBF was significantly and stably increased by PCF (10 ㎎/㎏, i.p.) during the period of cerebral reperfusion, which contrasted with the findings of rapid and marked increase in control group. In cytokine production in serum from femoral arterial blood 1 hr after middle cerebral arterial occlusion, PCF (10 ㎎/㎏, i.p.) significantly decreased IL-1β and TNF-α production, and increased IL-10 production compared with control group. In cytokine production in serum from femoral arterial blood 1 hr 1 hr after reperfusion, PCF (10 ㎎/㎏, i.p.) significantly decreased IL-1β production, and incresed IL-10 production compared with control group. These results suggested that PCF was significantly and stably increased regional cerebral blood flow by inhibiting IL-1β production, and by accelerating IL-10 production.

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목차

Ⅰ. 서론 = 1
Ⅱ. 재료 및 방법 = 2
1. 재료 = 2
1) 동물 = 2
2) 시료 = 2
2. 방법 = 2
1) 정상 흰쥐의 뇌혈류역학 변화 관찰 = 2
2) 정상 흰쥐의 뇌혈류역학 변화에 미치는 기전 관찰 = 3
3) In vitro 상에서 신경세포내 lactate dehydrogenase 활성도 측정 = 4
4) 뇌허혈 유발 흰쥐의 국소 뇌혈류량 변동에 미치는 개선 효과 관찰 = 5
5) 뇌허혈 유발 흰쥐의 혈청내 사이토카인 양 측정 = 6
3. 통계처리 = 8
Ⅲ. 성적 = 9
1. 정상 흰쥐의 국소 뇌혈류량 및 평균 혈압에 미치는 효과 = 9
2. 정상 흰쥐의 국소 뇌혈류량 작용기전 관찰 = 11
3. 정상 흰쥐의 평균 혈압 작용기전 관찰 = 13
4. 생쥐의 신경세포내 LDH 활성도에 미치는 효과 = 15
5 뇌허혈 유발 흰쥐의 국소 뇌혈류량 변동에 미치는 개선 효과 = 17
6. 뇌허혈 유발 흰쥐의 혈청에서 생성되는 사이토카인 양에 미치는 효과 = 19
1) 중대뇌동맥 폐색 1시간 후 생성되는 사이토카인 양에 미치는 효과 = 19
2) 재관류 1시간 후 생성되는 사이토카인 양에 미치는 효과 = 21
Ⅳ. 고찰 = 23
Ⅴ. 결론 = 30
참고 문헌 = 32

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