水蛭이 streptozotocin으로 유발된 rat의 糖尿病性 腎症에 미치는 影響
(The) effects of Hirudo on streptozotocin-induced diabetic nephropathic rats
- 주제(키워드) 수질 , Streptozotocin , 당뇨병
- 발행기관 東新大學校 大學院
- 발행년도 2007
- 학위명 박사
- 학과 한의학과
초록/요약
The aim of present study was to investigate recovery effects of Hirudo which has been used clinically in diabetes therapy. Mice were administered streptozotocin(STZ) to induce diabetes. The results were as follows: 1. The protein amount in urine per 24hrs of Hirudo treated group as compared to the control group was significantly reduced. 2. The BUN, creatinine level in serum of Hirudo treated group as compared to the control group was significantly inhibited. 3. The construction change in kidney of Hirudo treated group as compared to the control group was significantly inhibited. 4. The factor of Hirudo treated group as compared to the control group was significantly inhibited which induce the construction change in kidney. The above results suggest that Hirudo partially improved the function of the kidney.
more목차
目次
ABSTRACT = ⅰ
Ⅰ. 서론 = 1
Ⅱ. 재료 및 방법 = 2
1. 재료 = 2
1) 약재 = 2
2) 동물 = 2
3) 시약 및 기기 = 2
2. 방법 = 2
1) 검액의 제조 = 2
2) 당뇨병성 신증의 병태유발 = 3
3) 실험군의 배정 및 연구 과정 = 3
4) 24시간 요단백 배설량과 체중 측정 = 4
5) 혈액학적 분석 = 4
6) 신장의 조직병리학적 평가 = 4
7) Macrophage/monocyte antigen(ED-1), transforming growth factor(TGF-β1), Type Ⅳ collagen의 면역조직화학염색. = 5
8) Receptor for advancecd glycation end-product(RAGE)의 면역조직형광염색 = 6
9) TGF-β1 및 AGE의 ELISA측정 = 7
10) 통계 처리 = 8
Ⅲ. 실험결과 = 9
1. 24시간 요단백 배설량과 체중 측정 = 9
2. 혈청 중 각종 성분의 변화에 미치는 영향 = 12
3. 신장의 조직 병리학적 변화에 미치는 영향 = 13
4. Macrophage/monocyte antigen(ED-1), TGF-β1과 Type Ⅳ collagen의 면역조직화학염색의 변화에 미치는 영향 = 16
5. Receptor for advancecd glycation end-product(RAGE)의 면역조직형광염색의 변화에 미치는 영향 = 23
6. 혈청 advanced glycation end-product(RAGE)와 transforming growth factor(TGF-β1) 농도에 미치는 영향 = 26
Ⅳ. 고찰 = 28
Ⅴ. 결론 = 35
참고문헌 = 36