허혈성 뇌손상 흰쥐에서 인지 및 운동기능회복에 미치는 소풍탕의 효과
Neuroprotective effects of Sopung-tang on cognition and motor function recovery after ischemic brain injury in rats
- 주제(키워드) Sopung-tang , Ischemic brain injury , Congnition and motor function recovery , BDNF , Morris water maze
- 발행기관 동신대학교 대학원
- 지도교수 최진봉
- 발행년도 2008
- 학위수여년월 2008. 2
- 학위명 석사
- 학과 대학원 한의학과
- 세부전공 한방재활의학과
- 원문페이지 ⅲ, 49 p.
- 본문언어 한국어
초록/요약
Sopung-tang is a famous herbal prescription that treated ischemic brain injury. This study was designed to evaluate the effects of Sopung-tang on congnition and motor function recovery after ischemic brain injury in rats. Male rats were divided into 4 groups. Control groupⅠwas per os normal saline for 7 days after ischemic brain injury. Control group Ⅱ was per os normal saline for 14 days after ischemic brain injury. Experimental groupⅠ(Ex Ⅰ) was taken with Sopung-tang for 7 days after ischemic brain injury. Experimental group Ⅱ(Ex Ⅱ) was taken with Sopung-tang for 14 days after ischemic brain injury. The author carried out neurological, cognitive motor behavior tests and histological assessment. Neurological motor behavior tests consist of limb placement test, beam-walking test and horizontal wire test. Morris water maze test was used for cognitive motor behavior test. In the histological assessment test, TTC(2,3,5-triphenylteterazolium chloride) staining, Hematoxylin & Eosin staining and immunohistochemical staining were experimented. The results are as follows: 1. In neurological motor behavior tests, motor function recovery was significantly increased in the experimental groups as compared with control groups(p<0.05). Especially Ex Ⅱ was significantly increased as compared with Ex Ⅰ(p<0.05). 2. In Morris water maze test, congnitive motor function recovery was significantly incresed in the experimental groups as compared with control groups(p<0.05). Especially Ex Ⅱ was significantly increased as compared with Ex Ⅰ(p<0.05). 3. In the immunohistochemical staining for the expression of BDNF in hippocampus, more immune reaction was investigated in the experimental groups as compared with control groups. Especially Most immune reaction was experimented in the Ex Ⅱ. According to the above results, Sopung-tang can treat on the congnition and motor function recovery after ischemic brain injury in rats. And it is effective method in expression of BDNF in hippocampus.
more목차
ABSTRACT = i
Ⅰ. 서론 = 1
Ⅱ. 재료 및 방법 = 3
1. 실험 재료 및 약재 = 3
1) 실험 동물 = 3
2) 약재 = 5
2. 실험 방법 = 6
1) 허혈성 뇌손상 유발 = 6
2) 소풍탕 제조 및 투여 = 6
3. 결과측정 = 7
1) 신경학적 운동행동 검사 = 7
2) 인지적 운동행동 검사 = 8
3) 조직학적 검사 = 9
4. 통계처리 = 11
Ⅲ. 결과 = 12
1. 신경학적 운동행동 평가 = 12
1) 체지배치 검사 = 12
2) 막대걷기 검사 = 16
3) 철사잡기 검사 = 20
2. 인지적 운동행동 평가 = 24
1) 수중미로 검사 = 24
3. 조직학적 평가 = 29
1) TTC염색 = 29
2) 간과 신장 독성 평가 = 30
3) 면역조직화학 염색 = 34
Ⅳ. 고찰 = 36
Ⅴ. 결론 = 42
참고문헌 = 44