5/6 腎切除 白鼠에서 生地黃의 지연 투여가 신기능 보호와 사구체 경화 억제에 미치는 영향 및 관련 기전연구
The Renoportective Effect of Rehmannia and It's Related Mechanism in 5/6 Nephrectomy-induced Chronic Renal Failure Rats
- 주제(키워드) Rehmannia , Nephrectomy , Chronic renal failure
- 발행기관 동신대학교 대학원
- 지도교수 김용성
- 발행년도 2008
- 학위수여년월 2008. 2
- 학위명 박사
- 학과 대학원 한의학과
- 원문페이지 ii, 48 p.
- 본문언어 한국어
초록/요약
The aim of present study was to investigate the renoprotective effects of Rehmannia which clinically has been used in treatment for the chronic renal failure. The experimental chronic renal failure was induced by 5/6 nephrectomy, and Rehmannia was orally administrated during 8weeks from 4weeks after 5/6 nephrectomy. We analyzed the BUN, creatinine and 24 hours urine protein excretion to evaluate the renal function, and also analyzed the synthesis of protein and the expression of RNA of TGF-β1, type IV collagen, angiotensin II and angiotensin II type 1 (AT1) receptor in systemic blood, renal cortex using by ELISA, immunohistochemistry and RT-PCR. The results were as follows: 1. The protein amount in urine per 24 hrs of Rehmannia treated group as compared to the control group was significantly reduced. 2. The creatinine levels in serum of Rehmannia treated group as compared to the control group was significantly reduced. 3. The construction change in kidney of Rehmannia treated group as compared to the control group was significantly inhibited. 4. In immunohistochemistry the positive cells of type Ⅳ collagen and angiotensin Ⅱ in glomerulus of Rehmannia treated group as compared to control group were significantly reduced 5. The TGF-β1 levels in serum of Rehmannia treated group as compared to the control group was significantly reduced. 6. In RT-PCR, the levels of TGF-β1, AT1, type IV collagen mRNA in kidney were increased in control group. In the group treated with Rehmannia, the levels of those were decreased. The above results suggest that Rehmannia might improve the function of the kidney.
more목차
I. 서론 = 1
Ⅱ. 재료 및 방법 = 3
1. 재료 = 3
1) 약재 = 3
2) 동물 = 3
3) 시약 및 기기 = 3
2. 방법 = 4
1) 검체의 제조 = 4
2) 5/6 신절제 만성 신부전 동물 모델의 제작 = 4
3) 실험군의 배정 및 연구 과정 = 5
4) 체중 및 24시간 요중 단백 측정 = 5
5) 혈액학적 분석 = 6
6) 신장의 조직병리학적 평가 = 6
7) Transforming growth factor-β1, type IV collagen, angiotensin II의 면역조직화학염색 = 7
8) 신장 조직에서의 RNA 분리 = 8
9) TGF-β1, collagen IVα1 및 angiotensin II type 1(AT1) receptor의 역전사 연쇄중합반응 = 8
10) Angiotensin II, TGF-β1의 ELISA측정 = 9
11) 통계 처리 = 10
Ⅲ. 성적 = 11
1. 체중과 24시간 요단백 배설량의 변화 = 11
2. 혈청 생화학 분석상 지표들의 변화 = 15
3. 혈액 분석상 지표들의 변화 = 18
4. 신장의 조직 병리학적 변화 = 19
5. 사구체 TGF-β1과 type IV collagen 및 angiotensin II의 면역조직화학염색상 변화 = 22
6. 혈청 TGF-β1과 angiotensin II 농도의 변화 = 26
7. 사구체 AT1 receptor, type IV collagen과 TGF-β1 유전자 발현의 변화 = 29
Ⅳ. 고찰 = 32
Ⅴ. 결론 = 41
참고문헌 = 43