皂角刺가 악성흑색종세포주에 미치는 영향
Pathways Analysis of Gleditsia spina Extract on Changes of Gene Expression in Human Melanoma cells
- 발행기관 동신대학교 대학원
- 지도교수 김용성
- 발행년도 2009
- 학위수여년월 2009. 2
- 학위명 석사
- 학과 대학원 한의학과
- 원문페이지 31 p.
- 본문언어 한국어
- 저작권 동신대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Glenditsia spina (GS) can resolve carbuncle, relive swelling, dispel wind and destroy parasites. For these reasons, GS has been widely used as dermatologic agent clinically. In this study, the specific pathways of anti-proliferative effect of GS on human derived melanoma cells were identified. The molecular profile was measured using microarray technique to identify up- or down-regulated genes in SK-MEL-2 cell line. Pathway analysis was done by listing percentage of pathway involvement, and the represented pathways were obtained from KEGG. The transcription factor binding sequences were obtained by Transfac database. By the promoter analysis, up-regulated genes by GS were mainly associated with MAPK, Regulation of actin cytoskeleton, Wnt, Focal adhesion and Long term potentiation pathway. Down-regulated genes by GS were mainly associated with MAPK and Antigen processing and presentation pathway. And some of the transcription factors like Sp1 and NF-Y in up-regulated genes and Oct-1 in down-regulated genes by GS also identified.
more목차
Ⅰ. 서론 = 1
Ⅱ. 재료 및 방법 = 2
1. 재료 = 2
1) 세포주 = 2
2) 약재의 준비 = 2
3) 시약 및 기기 = 2
2. 방법 = 2
1) 세포주 배양 환경 = 2
2) 약재 추출 = 3
3) RNA 분리를 위한 약물 처리 및 실험군 분류 = 3
4) RNA 분리, 정도 관리 및 DNA 칩을 이용한 실험 = 4
5) Data 분석 = 4
Ⅲ. 성적 = 7
1. 발현이 증가된 유전자들이 연관된 pathway 분석 = 7
2. 발현이 감소된 유전자들이 연관된 pathway 분석 = 14
3. 발현 차이를 보이는 유전자간의 pathway 비교 = 18
4. 전사인자 분석 = 23
Ⅳ. 고찰 = 25
Ⅴ. 결론 = 28
참고문헌 = 29