봉독약침과 녹용약침이 MIA 유도 골관절염 흰쥐에 미치는 영향
Effects of Bee Venom and Cervi Cornu Parvum Pharmaco Acupuncture in Monosodium iodoacetate(MIA)-induced osteoarthritis rat
- 주제(키워드) Bee Venom Pharmaco Acupuncture , Cervi Cornu Parvum Pharmaco Acupuncture , Monosodium iodoacetate(MIA)-induced osteoarthritis rat
- 발행기관 동신대학교 한의과대학원
- 지도교수 최진봉
- 발행년도 2009
- 학위수여년월 2009. 8
- 학위명 박사
- 학과 대학원 한의학과
- 세부전공 한방재활의학과
- 원문페이지 ⅱ, 50 p.
- 실제URI http://www.dcollection.net/handler/dsu/000000496772
- 본문언어 한국어
- 저작권 동신대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Objectives : The purpose of this study was to investigate the effects of Bee Venom (BV) and Cervi Cornu Parvum pharmaco acupuncture (CC) in monosodium iodide induced arthritis rats. Methods : The subjects were divided into 5 groups ; Normal, Control (no treatment after MIA), BV (Bee Venom pharmaco acupuncture 100 ㎕ daily at Dokbi (ST35) after inducing MIA), CC (Cervi Cornu Parvum pharmaco acupuncture 100 ㎕ daily at Dokbi (ST35) after inducing MIA) and BV+CC (Bee Venom pharmaco acupuncture and Cervi Cornu Parvum pharmaco acupuncture 100 ㎕ daily at Dokbi (ST35) after inducing MIA). After each operation, the present author observed the motor behavior recovery, hematological (Prostaglandin E2, AST, ALT), histological and immunological changes. Rats were tested at the 7th, 14th and 21th day. Results : Results are as follows. 1. All the experimental groups were improved compared with control group in plantar test. 2. All the experimental groups were improved compared with control group in touch test for sensory evaluator. 3. All the experimental groups were significantly decreased compared with control group in Prostaglandin E2. 4. In histological observations, knee joint in all the experimental groups were improved compared with control group. 5. In immunological observations, all the experimental groups were significantly decreased compared with control group in COX-1, 2. Conclusions : It can be suggested that Bee venom and Cervi Cornu Parvum pharmaco acupuncture may improve motor behavior, hematological, histological and immunological findings in MIA-induced osetoarthritis rats. Especially, combination of these two treatments will be somewhat better in osteoarthritis recovery and motor function improvement.
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ABSTRACT ⅰ
Ⅰ. 서 론 1
Ⅱ. 실 험 3
1. 재료 3
1) 동물 3
2) 군 분류 3
2. 약침액 및 모델 5
1) 약침액의 제조 5
2) 약침 혈위 선정 및 약침 시술 방법 5
3) 관절염 모델 5
3. 평가방법 6
1) 족저부 통증 평가 6
2) 접촉 감각 평가 6
3) 혈청에서 PGE2 농도 측정 7
4) 혈청학적 검사 8
5) 병리조직학적 평가 및 특수염색 8
(1) 조직처리 8
(2) Hematoxylin & Eosin 염색 9
(3) Safranin O-fast green 염색 9
(4) 조직학적 관찰 및 평가 9
6) 면역조직화학 염색 11
4. 통계처리 12
Ⅲ. 성 적 13
1. 봉독약침과 녹용약침의 안전성 및 MIA 안정성 평가 13
1) 간 독성에 미치는 영향 13
2) 간과 신장의 병리조직학적 평가 16
3) MIA로 유도된 퇴행성관절염의 조직학적 소견 20
2. 봉독약침과 녹용약침의 유효성 평가 23
1) 족저부 통증 평가 23
2) 접촉 감각 평가 25
3) 혈중 PGE2 농도에 미치는 영향 28
4) 병리조직학적 평가 31
5) COX-1의 면역조직화학적 변화 35
6) COX-2의 면역조직화학적 변화 38
Ⅳ. 고 찰 41
Ⅴ. 결 론 46
참고문헌 47