中性瘀血 藥鍼과 當歸鬚散加味湯이 외상성 뇌손상 흰쥐의 신경보호에 미치는 영향
Neutral Pharmacopuncture for Static Blood and Tangguisusangamitang on Neuroprotective effect in the Experimental Traumatic Brain Injury Rats
- 주제(키워드) Neutral Pharmacopuncture for Static Blood , Tangguisusangamitang , SP10 , BAX and BCL-2 expression , TINEL staining , Neurological motor behavioral test
- 발행기관 동신대학교 한의과대학원
- 지도교수 최진봉
- 발행년도 2009
- 학위수여년월 2009. 9
- 학위명 박사
- 학과 대학원 한의학과
- 세부전공 한방재활의학과
- 원문페이지 ⅲ, 45 p.
- 실제URI http://www.dcollection.net/handler/dsu/000000496773
- 본문언어 한국어
- 저작권 동신대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Neutral Pharmacopuncture for Static Blood and Tangguisusangamitang are famous treatment that treated disease caused by Static Blood. Hyolhae(SP10) is also a famous point of acupuncture that treated that. This study was designed to evaluate the effects of Neutral Pharmacopuncture for Static Blood into Hyolhae(SP10) and Tangguisusangamitang on Neuroprotective effect in the experimental Traumatic Brain Injury(TBI) rats. Male rats were divided into 4 groups. Group Ⅰ was no treatment after TBI. Group Ⅱ was treatment with Neutral Pharmacopuncture for Static Blood into Hyolhae(SP10) after TBI. Group Ⅲ was treatment with Tangguisusangamitang after TBI. Group Ⅳ was Neutral Pharmacopuncture for Static Blood into Hyolhae(SP10) and Tangguisusangamitang after TBI. The author carried out neurological motor behavioral test, histological assessment test. Neurological motor behavior tests consist of rotarod test, beam-walking test and postural reflex test. In the histological assessment test, BAX and BCL-2 expression, Hematoxylin & Eosin staining, TUNEL staining were experimented. The results are as follows: 1. In neurological motor behavior tests, motor and cognitive function recovery was significantly increased in the Group Ⅱ, Ⅲ, Ⅳ as compared with Group Ⅰ. also Group Ⅳ was increased as compared with each Group Ⅱ, Ⅲ. Especially Group Ⅳ was significantly increased as compared with Group Ⅱ. 2. In BAX expression, according to priority Group Ⅳ, Ⅲ, Ⅱ, Ⅰ were decresed in 7 and 14 days later. Especially Group Ⅳ was significantly decreased in 14days later. 3. In BCL-2 expression, Group Ⅳ was increased slightly in 7days later. Group Ⅲ, Ⅱ were increased slightly in 14days later. Most incresed expression was experimented in the Group Ⅳ in 14days later. 4. In TUNEL expression, Ⅳ was decreased as compared with each Group Ⅰ,Ⅱ, Ⅲ in 7days later. Group Ⅳ, Ⅲ were decreased as compared with each Group Ⅰ,Ⅱ in 14days later. According to the above results, Neutral Pharmacopuncture for Static Blood and Tangguisusangamitang can inhibit apoptosis of cells after TBI in rats by contol of BAX and BCL-2, TUNEL expression. And also can help neurological motor behavioral function.
more목차
ABSTRACT i
Ⅰ. 서 론 1
Ⅱ. 재료 및 방법 3
1. 동물 및 약재 3
1) 동물 3
2) 약재 5
2. 방법 5
1) 검액의 제조 5
2) 中性瘀血 藥鍼의 제조 6
3) 외상성 뇌손상 유발 7
4) 當歸鬚散加味湯의 투여 및 약침의 시술 7
3. 결과측정 7
1) 조직학적 검사 7
2) 신경학적 운동행동 검사 10
3) 조직학적 분석 11
4. 통계처리 12
Ⅲ. 성 적 13
1. 조직학적 검사 13
1) H & E와 cresyl violet 염색 13
2) 독성 평가 14
(1) 간독성 검사 14
(2) 신장독성 검사 16
2. 신경학적 운동행동 평가 18
1) Rotarod 검사 18
2) Beam-walking 검사 20
3) Postural reflex 검사 22
3. 면역조직화학 염색 24
(1) BAX 염색 24
(2) BCL-2 염색 28
(3) TUNEL 염색 31
Ⅳ. 고 찰 34
Ⅴ. 결 론 39
참 고 문 헌 41