죽엽이 생쥐의 복강암에 미치는 영향
- 발행기관 동신대학교 한의과대학원
- 지도교수 박수연
- 발행년도 2009
- 학위수여년월 2009. 8
- 학위명 석사
- 학과 대학원 한의학과
- 실제URI http://www.dcollection.net/handler/dsu/000000496927
- 본문언어 한국어
- 저작권 동신대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
ABSTRACT Effects of Phyllostachyos Folium (PF) on solid tumor in mice Song Jin Soo Dept. of Oriental Medicine Graduate School of Dongshin University (Directed by Prof. Park Soo Yeon, O.M.D., Ph. D.) Phyllostachyos Folium (PF) has been used to treat patients with febrile disease consuming the body fluids marked by fever with restlessness, thirst etc. In the theory of herbology, PF can clear away heat and promote the production of the body fluids, relieve restlessness. Recently PF is known to have anti-bacterial, anti-oxidantic effects. The present study was carried out to investigate the effects of PF on solid tumor in mice in terms of immune-potentiating and direct cytotoxic action of PF in vitro and vivo study. The present author investigated thymocyte and splenocyte proliferation to confirm immune-potentiating activity of PF and also investigated tumor/body weight ratio and survival rates in tumor bearing mice. In this study, administration of PF decreased tumor/body weight ratio significantly, and prolonged survival duration compared to non-treated control. In addition, treatment with PF suppressed proliferation rate of Sarcoma 180 (S-180) cells significantly, and elevated proliferation rates of thymocytes isolated from normal mice. These results were co-related with in vivo study. In conclusion, these results suggest that PF is useful to treat patient with solid tumor, because PF has direct toxic action for tumor cell and immune -potentiating action for T cells. Further study will need to elucidate exact mechanisms related in anti-cancer action of PF.
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目 次
ABSTRACT
Ⅰ. 서 론 1
Ⅱ. 재료 및 방법 3
1. 재료 3
1) 세포주 및 배양환경 3
2) 실험 동물 3
3) 약재 3
4) 시약 및 기기 4
2. 방법 5
1) 시료의 제조 5
2) 복강암의 유발 및 실험군 선정 5
3) 복강암의 무게 및 체중 측정 5
4) 생존율 관찰 5
5) 면역세포 분리 6
6) S-180 복강암 세포주 및 면역세포의 증식률 측정 6
3. 통계처리 7
Ⅲ. 성 적 8
1. 암종이 유발된 생쥐의 체중에 미치는 영향 8
2. 암종이 유발된 생쥐의 암종/체중 비율에 미치는 영향 10
3. 암종이 유발된 생쥐의 생존율에 미치는 영향 14
4 S-180 복강암 세포주의 증식률에 미치는 영향 17
5. 흉선 세포의 증식률에 미치는 영향 19
6. 비장 세포의 증식률에 미치는 영향 21
Ⅳ. 고 찰 23
Ⅴ. 결 론 28
참 고 문 헌 29