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枇杷葉 에탄올 추출물이 HaCaT keratinocyte 에 미치는 항산화 효과

초록/요약

ABSTRACT Effects of Eriobotryae Folium as Anti-Oxidant on HaCaT keratinocyte Park Yoon Hee Dept. of Oriental Medicine Graduate School of Dongshin University (Directed by Prof. Park Soo Yeon, O.M.D., Ph. D.) Eriobotryae Folium (EF) has been used to treat patients with cough in oriental countries. In the theory of oriental medicine, EF can clear away lung-heat, resolve phlegm, reliveve cough and lower the adverse-rising qi and arrest vomiting. Recently, EF is known to have anti-oxidantic action. For these reasons, the present study designed this study to investigate anti-oxidative effects of EF on HaCaT keratinocyte. The present study measured the amount of polyphenoics and flavonoids, and also measured the levels of catalase, Ascorbate peroxidase (APX), SOD like activities and DPPH free radical scavenging activity. Then the effects of SB on viability and prolferation rates, and protective effects against oxidative stress induced by chemicals such as hydrogen peroxide and rotenone were also investigated. In this experiment, treatment with EF did not show cytotoxicity on HaCaT keratinocyte below the concentration of 100 ㎍/㎖. Especially, treatment with 50 ㎍/㎖ and 100 ㎍/㎖ of EF elevated proliferation rates of HaCaT keratinocyte significantly. 96.8±1.8 ㎍/㎖ of total phenolics and 53.2±1.6 ㎍/㎖ of flavonoids was detected from fresh EF ethanol extract. In addition, DPPH free radical scavenging activities of EF were elevated in dose-dependent manner. Catalase and APX activities were 6.3±0.2 and 21.2±4.6 U/mg protein/min respectively. Finally, EF showed protective effect against cell death of HaCaT keratinocyte induced by rotenone and SNP significantly. In conclusion, these results suggest that EF may have anti-oxidantic action in human skin and also suggest that EF can be used as anti-aging agent.

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목차

목 차

ABSTRACT ⅰ

Ⅰ. 서 론 1

Ⅱ. 실험재료 및 방법 2
1. 재료 2
1) 약재 2
2) 시약 및 기기 2
3) 세포주 2
2. 실험 방법 2
1) 시료의 추출 2
2) 세포 배양 3
3) 세포 생존율 및 증식율에 미치는 영향 3
4) 총 페놀 함량 측정 3
5) 총 플라보노이드 함량 측정 4
6) DPPH radical 소거 활성 측정 5
7) SOD like activity 측정 5
8) 항산화 효소활성 측정을 위한 효소액 조제 6
9) Catalase (CAT)활성 측정 6
10) Ascorbate peroxidase(APX)활성 측정 6
11) 4종 산화적 스트레스 요인에 의한 세포 손상 보호 효과 측정 6

3. 통계처리 9



Ⅲ. 실험 성적 10
1. 세포 생존율에 미치는 영향 10
2. 세포 증식율에 미치는 영향 11
3. 총 폐놀류 함량 12
4. 총 플라보노이드류 함량 13
5. DPPH 라디칼 소거 활성 14
6. SOD like activity 15
7. Catalase (CAT)활성 16
8. Ascorbate peroxidase(APX)활성 17
9. 과산화수소에 의하여 발생하는 산화적 스트레스에 대한 보호효과 18
10. Rotenone에 의하여 발생하는 산화적 스트레스에 대한 보호효과 19
11. Paraquat에 의하여 발생하는 산화적 스트레스에 대한 보호효과 20
12. Sodium nitroprusside (SNP)에 의하여 발생하는 산화적 스트레스에 대한 보호효과 21

Ⅳ. 고찰 22

Ⅴ. 결론 29

참고문헌 31

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