Scopolamine으로 유발된 치매 白鼠 모델에서 歸脾湯의 인지기능 및 기억력 향상에 미치는 효과
The enhancing effects of Gwibi-tang(guīpí-tāng) on cognitive function and memory in scopolamine-induced dementia rat model
- 주제(키워드) 치매 , 귀비탕 , Scopolamine
- 발행기관 동신대학교 대학원
- 지도교수 김선종
- 발행년도 2011
- 학위수여년월 2011. 2
- 학위명 석사
- 학과 및 전공 대학원 한의학과
- 세부전공 한방재활의학과
- 원문페이지 52P
- 실제URI http://www.dcollection.net/handler/dsu/000000892563
- 본문언어 한국어
- 저작권 동신대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Objectives : The water extract of Gwibi-tang has been traditionally used for treatment of psychologic disease and brain damage in oriental Medicine. The purpose of this experiment was designed to investigate the effects of Gwibi-tang on dementia induced by scopolamine in the rats. Methods : Groups were divided into 4 groups ; Normal group, Control group, Positive Control Group(tacrine group) and Gwibi-tang group. Control group: To induce dementia, 1mg/kg of Scopolamine intraperitoneal injection is used for 14 days(1 per day) in the rats. Positive Control Group(Tacrine group): 10 mg/kg of Tacrine, the dementia treatment, is medicated in oral inoculation. After 30 mintues, 1mg/kg of scopolamine intraperitoneal injection, which causes dementia, is used for 14 days(1 per day). Gwibi-tang group: 300 mg/kg of Gwibi-tang is medicated in oral inoculation. After 30 mintues, 1mg/kg of scopolamine intraperitoneal injection, which causes dementia, is used for 14 days(1 per day). The present author observed the passive avoidance performance test, the spatial learning and memory ability test(Morris water maze test), the values of acetylcholine, Acetlycholin Esterase(AchE) activity in the hippocampal, the cerebral cortex. Results : 1. In passive avoidance test on 1st day and 3rd day of causing dementia, Gwibi-tang group appeared to be more significant than Positive control group. On 7th day and 14th day, Gwibi-tang group appeared to be more significant than normal control group and control group. However, there was no significant difference between Gwibi-tang group and Positive control group. 2. In a test of spatial learning and memory ability, on 7th day and 14th day of causing dementia, Gwibi-tang group and Positive control group appeared to be more significant more than control group. There was no significant difference between Gwibi-tang group and Positive control group. 3. In Ach and AchE activity test, Ach and AchE activity in brain system and the hippocampal, cerebral cortex appeared to be more significant in Gwibi-tang group and Positive control group than control group. However, there was no significant difference between Gwibi-tang group and Positive control group. 4. In liver and kidney tissue tests, all experimental group appeared to be normal. which indicates that Gwibi-tang does not damage liver and kidney. From the results above, Gwibi-tang is thought to be effective in improvement of memory related to cholinergic nerve problem and dementia. Conclusions : The present author thought that Gwibi-tang might be usefully applied for prevention and treatment of dementia and memory disorder. Further study is needed to conduct a rigorous clinical research.
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ABSTRACT
Ⅰ. 서론 1
Ⅱ. 재료 및 방법 3
1. 실험동물 및 약재 3
1) 실험동물 3
2) 약재 3
3) 검액제조 4
2. 실험방법 5
1) 군 분류 5
2) 측정항목 6
3) 수동회피검사(passive avoidance performance test) 6
4) 공간지각 학습능력 검사(Spatial learning and memory ability test) 7
5) 생화학적 변화 검사 8
3. 통계처리 및 분석 9
Ⅲ. 결과 10
1. 수동회피실험에 미치는 효과 10
2. 공간지각 학습능력에 미치는 효과 13
3. 생화학적 변화에 미치는 효과 18
1) ACh 함량 변화 18
2) AChE 활성도 변화 21
4. 조직학적 검사 24
1) 간독성검사 24
2) 신장독성검사 26
Ⅳ. 고찰 27
Ⅴ. 결론 35
참고문헌 37