육미지황탕과 녹용약침이 당뇨유발 백서의 골절치유에 미치는 영향
Effects of Yukmijihwang-tang(Liuweidihuang-tang) and Cervi Pantotrichum Cornu Pharmacopuncture on fracture healing in Diabetic Rats.
초록/요약
ABSTRACT Effects of Yukmijihwang-tang(Liuweidihuang-tang) and Cervi Pantotrichum Cornu Pharmacopuncture on fracture healing in Diabetic Rats. Kee Young-Bum Dept. of Oriental Medicine Graduate School of Dongshin University (Directed by Prof. Choi Jin-Bong, O.M.D., Ph. D.) Objectives : This study was designed to evaluate the effects of Yukmijihwang-tang(Liuweidihuang-tang) and Cervi Pantotrichum Cornu Pharmacopuncture on fracture healing in Diabetic Rats. Methods : We divided the subjects into 4 groups : Control group with no treatment, Experimental Ⅰ taken with Yukmijihwang-tang(Liuweidihuang-tang), Experimental Ⅱ taken with Cervi Pantotrichum Cornu Pharmacopuncture and Experimental Ⅲ taken with Yukmijihwang-tang(Liuweidihuang-tang) and Cervi Pantotrichum Cornu Pharmacopuncture groups. Serum T-ALP, Histological observation of callus formation, and the degree of TGF-β1 in each group were observed at the 7th, 14th, 21th day and at the 28th day respectively. Results : 1. Experimental group Ⅰ and Experimental group Ⅲ showed significant changes in serum T-ALP after 7-days of experimental period. All the experimental groups showed significant changes in serum T-ALP after 14-days of experimental period, Especially experimental group Ⅲ showed more significant changes than other groups. 2. After 14-days, 21-days and 28-days, all the experimental groups showed significant changes in histological observation of callus formation, compared with control group. Especially experimental group Ⅲ showed more significant changes than other groups. 3. All the experimental groups showed significant changes in TGF-β1 compared with control group. Especially experimental group Ⅲ showed more significant changes than other groups. 4. Injury of liver tissue and kidney tissue in histological findings were not seen after 28-days of experimental period. Conclusions: It is suggested that Yukmijihwang-tang(Liuweidihuang-tang) and Cervi Pantotrichum Cornu Pharmacopuncture may play a significant role in fracture healing in Diabetic Rats, and these two treatments could be more effective when used together. Key words : Yukmijihwang-tang(Liuweidihuang-tang), Cervi Pantotrichum Cornu Pharmacopuncture, Fracture, Diabetic Rats
more목차
ABSTRACT 1
Ⅰ. 서 론 3
Ⅱ. 연구방법 5
1. 실험동물 5
2. 당뇨병 유발 6
3. 당뇨병 유발 여부 판별 6
4. 골절 유발 6
5. 육미지황탕 경구투여 6
6. 녹용약침 시술 7
7. T-ALP(Total Alkaline Phosphatase)의 혈액학적 분석 7
8. 조직절편의 제작 7
1) 골절 부위의 형태학적 관찰 8
2) 간과 신장 독성 검사 8
9. TGF-β1에 대한 면역조직화학법 8
10. 통계처리 9
Ⅲ. 결과 10
1. T-ALP의 변화 10
2. 골절 유발 후 골절 치유에 대한 형태학적 관찰 12
3. 가골에서 TGF-β1에 대한 면역조직화학법 17
4. 간, 신장 독성 검사 23
1) 간독성 검사 23
2) 신장독성 검사 24
Ⅳ. 고 찰 25
Ⅴ. 결 론 30
Ⅵ. 참고문헌 31