三七根이 만성 신부전에 미치는 영향
- 주제(키워드) 신부전
- 발행기관 동신대학교 한의과대학원
- 발행년도 2011
- 학위수여년월 2011. 2
- 학위명 박사
- 학과 및 전공 대학원 한의학과
- 실제URI http://www.dcollection.net/handler/dsu/000000945441
- 본문언어 한국어
- 저작권 동신대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
ABSTRACT Effects of Panax notoginseng saponins on chronic renal failure. Kwun-Ho Lee Dept. of Oriental Medicine Graduate School of Dongshin University Directed by Prof. Young-Seung, Kim, O.M.D., Ph. D. The aim of present study was to investigate recovery effects of Panax notoginseng saponins(Panax) which has been used clinically in chronic renal failure therapy. Mice had 5/6 nephrectomy to induce chronic renal failure. The results were as follows: 1. The protein amout in urine per 24hrs of Panax treated group as compared to the control group was significantly reduced. 2. The albumin and HDL-cholesterol amout in blood of Panax treated group as compared to the control group significantly increased. The creatinine level in blood of Panax treated group as compared to the control group was significantly inhibited. 3. The construction change in kidney of Panax treated group as compared to the control group was significantly inhibited. 4. The factor(TGF-β1, type IV collagen, Macrophage/monocyte antigen(ED-1) and angiotensin II type 1 receptor(AT1)) of Panax treated group as compared to the control group was significantly inhibited which induce the construction change in kidney. The above results suggest that Panax partially improved the function of the kidney. Key words : Panax notoginseng saponins, nephrectomy, chronic renal failure
more목차
ABSTRACT ⅰ
Ⅰ. 서론 1
Ⅱ. 재료 및 방법 3
1. 재료 3
1) 동물 3
2) 약재의 준비 3
3) 시약 및 기기 3
2. 방법 4
1) 검체의 제조 4
2) 5/6 신절제 만성 신부전 동물 모델의 제작 4
3) 실험군의 배정 및 연구 과정 5
4) 체중 및 24시간 요중 단백 측정 5
5) 혈액학적 분석 5
6) 腎臟의 조직병리학적 평가 6
7) Macrophage/monocyte antigen(ED-1), TGF-β1, type IV collagen, angiotensin II type 1 receptor(AT1)의 면역조직화학염색 6
8) Angiotensin II type 1 receptor(AT1) 및 angiotensin converting enzyme(ACE)의 RT-PCR 7
9) 통계 분석 8
Ⅲ. 결과 10
1. 체중과 24시간 요단백 배출량에 미치는 영향 10
2. 혈청 생화학 분석상 지표들의 변화에 미치는 영향 11
3. 腎臟의 조직 병리학적 변화에 미치는 영향 14
4. TGF-β1과 type IV collagen, macrophage/monocyte antigen(ED-1) 및 angiotensin II type 1 receptor(AT1)의 면역조직화학염색의 변화에 미치는 영향 16
5. 사구체 angiotensin II type 1 receptor(AT1) 및 angiotensin converting enzyme(ACE) 유전자 발현에 미치는 영향 21
Ⅳ. 고찰 23
Ⅴ. 결론 30
참고문헌 31