길경추출물이 C6 glioma cell의 산화적스트레스에 대한 세포사멸 보호효과
The Effect of Platycodon Grandiflorum extract(PGE) on oxidative stress in C6 glioma cell apoptosis protective.
- 주제(키워드) 길경추출물 , C6 glioma cell , 산화적스트레스
- 발행기관 동신대학교 대학원
- 지도교수 전상윤
- 발행년도 2014
- 학위수여년월 2014. 2
- 학위명 석사
- 학과 및 전공 대학원 한의학과
- 원문페이지 34
- 실제URI http://www.dcollection.net/handler/dsu/000001675358
- 본문언어 한국어
- 저작권 동신대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
ABSTRACT The Effect of Platycodon Grandiflorum extract(PGE) on oxidative stress in C6 glioma cell apoptosis protective. Jae Joon Jo Dept. of Oriental Medicine Graduate School of Dongshin University Directed by Prof. Sang Yoon Jeon, O.M.D, Ph.D. This study is designed to investigate the effects of Platycodon Grandiflorum extract (PGE) by measuring the changes of the protective effects on cell death induced by chemicals such as paraquat, SNP, Rotenone, hydrogen peroxide and anti-oxidative effects in C6 glioma cells. The results are as follows ; 1. Platycodon Grandiflorum extract 250 ㎍ / ㎖ significantly above the level of concentration and reduced cell proliferation. 2. In case of administration platycodon grandiflorum extract, Paraquat been induced by apoptosis is significantly decreased. 3. In case of administration platycodon grandiflorum extract, SNP been induced by apoptosis is significantly decreased. 4. In case of administration platycodon grandiflorum extract, H2O2 been induced by apoptosis is significantly decreased. 5. In case of administration platycodon grandiflorum extract, Rotenone been induced by apoptosis is decreased, but the statistical significance is not. 6. In case of administration platycodon grandiflorum extract, Activity of SOD significantly increased. 7. In case of administration platycodon grandiflorum extract, Total glutathione did not affect the content. In conclusion, our study suggests that PGE is a disease caused by oxidative stress and aging, the prevention and treatment of food shall be able to be applied. Key words : Platycodon Grandiflorum extract(PGE), C6 glioma cell, Oxidative stress
more목차
목 차
ABSTRACT ⅰ
Ⅰ. 서 론 1
Ⅱ. 재료 및 방법 3
1. 재료 3
1) 세포주 3
2) 약재 3
2. 방법 3
1) 시료의 제조 3
2) 세포 배양 3
3) 세포 증식율 측정 4
4) 산화적 스트레스에 의한 세포 사멸 보호효과 측정 4
5) 실험군 설정 5
6) SOD 활성 측정 7
7) Total glutathione 함량 측정 7
3. 통계처리 7
Ⅲ. 성 적 9
1. 세포 증식율에 미치는 영향 9
2. 산화적 스트레스에 의한 세포 사멸 11
3. Paraquat에 의해 발생한 세포사멸 방지효과 13
4. SNP에 의해 발생한 세포사멸 방지효과 15
5. Rotenon에 의해 발생한 세포사멸의 방지효과 17
6. Hydrogen peroxide에 의해 발생한 세포사멸의 방지효과 19
7. SOD 활성에 미치는 영향 21
8. Total glutathione 함량에 미치는 영향 23
Ⅳ. 고 찰 25
Ⅴ. 결 론 29
Ⅵ. 참 고 문 헌 30