희첨 추출물이 B16F10 세포에서 Melanin 생성과 Tyrosinase 활성에 미치는 억제효과
Inhibitory Effects of Siegesbeckia Herba Extracts on the Melanin Production and Tyrosinase Activity in B16F10 cells
- 주제(키워드) Siegesbeckia , Melanin , Tyrosinase , B16F10
- 발행기관 동신대학교 대학원
- 지도교수 박수연
- 발행년도 2015
- 학위수여년월 2015. 2
- 학위명 석사
- 학과 및 전공 대학원 한의학과
- 원문페이지 29
- 실제URI http://www.dcollection.net/handler/dsu/000001864939
- 본문언어 한국어
- 저작권 동신대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Siegesbeckia Herba can treat various skin disease by expelling wind and removing dampness and clearing away heat and toxic material effects. This study was designed to investigate effects of Siegesbeckia Herba Extracts(SHE) on skin elasticity and whitening using B16F10 cell lines. In this experiment, We observed effect of SHE on cell viability, inhibition of melanin synthesis and inhibitory effect on tyrosinase and elastase. In results, SHE treated group showed decreased cell viability rates significantly compared with non-treated group. More than SHE 250 ㎍/㎖, 500 ㎍/㎖ and 1,000 ㎍/㎖ of treated groups were lower levels of melanin synthesis respectively. SHE significantly showed tyrosinase inhibitory activity in vitro, SHE increased tyrosinase inhibitory activity and elastase inhibitory activity in B16F10 cells, and tyrosinase inhibitory activity in vitro. But tyrosinase inhibitory activity and elastase inhibitory activity in B16F10 cells, tyrosinase inhibitory activity in vitro were not accepted statistical significance compared with non-treated group. And SHE treated group showed increased SOD-like activity rates significantly compared with non-treated group. More than SHE 250 ㎍/㎖, 500 ㎍/㎖ and 1,000 ㎍/㎖ of treated groups were lower levels of melanin synthesis respectively. In conclusion, these results suggest that SHE can inhibit melanin synthesis and tyrosinase inhibtory activity. So, We suggest that SHE can be maintained skin whitening.
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目 次
ABSTRACT i
Ⅰ. 서 론 1
Ⅱ. 재료 및 방법 2
1. 재료 2
1) 약재 2
2) 세포주 2
2. 방법 2
1) 시료의 추출 2
2) 세포 배양 2
3) 세포 생존율 측정 3
4) Melanin 생성 억제율 측정 3
5) 비세포의 tyrosinase 저해 활성 측정 4
6) 세포 내 tyrosinase 저해 활성 측정 4
7) 비세포의 elastase 저해 활성 측정 5
8) 세포 내 elastase 저해 활성 측정 5
9) Superoxide dismutase(SOD) 유사활성 측정 6
3. 통계처리 7
Ⅲ. 성 적 8
1. 세포 생존율에 미치는 효과 8
2. Melanin 생성에 미치는 억제 효과 10
3. 비세포의 tyrosinase 저해 활성 12
4. 세포 내 tyrosinase 저해 활성 14
5. 비세포의 elastase 저해 활성 15
6. 세포 내 elastase 저해 활성 16
7. SOD 유사활성 17
Ⅳ. 고 찰 19
Ⅴ. 결 론 24
참 고 문 헌 25