황칠이 만성 스트레스 유발 백서의 스트레스 및 수면 호르몬에 미치는 영향
Effect of Resina Dendropanacis morbiferus on Stress and Sleep Hormone in Chronic Mild Stress-Induced Rats
- 주제(키워드) Resina Dendropanacis morbiferus(RDM) , Chronic Mild Stress(CMS) , Stress Hormone , Insomnia , Noradrenalin , GABA , Melatonin , Animal model
- 발행기관 동신대학교
- 지도교수 김경옥
- 발행년도 2017
- 학위수여년월 2017. 2
- 학위명 박사
- 학과 및 전공 대학원 한의학과
- 실제URI http://www.dcollection.net/handler/dsu/000002334038
- 본문언어 한국어
- 저작권 동신대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
OBJECTIVES : This study aimed to evaluate the effects of Resina Dendropanacis morbiferus(RDM) on stress and sleep hormones when administered to Chronic Mild Stressed(CMS) rats. METHODS : 25 healthy Rats were randomly diveded into 5 groups ; Normal, CMS(Control), Resina Dendropanacis mobiferus 50 mg/kg(RDM 50), 100 mg/kg(RDM 100), 200 mg/kg(RDM 200). All rats except the normal group were exposed to unpredicted stress condition like water deprivation, empty bottles, forced threadmill, and etc. according to the timetable of CMS for 3 weeks. After a week starting experiment, Rats in RDM 50, RDM 100, and RDM 200 groups were fed orally once a day for 2 weeks. Afterward, Blood samples were taken from the rats to analyze Complete blood count, AST, ALT, and Glucose. Noradrenalin, GABA and Melatonin were measured by ELISA kit. BDNF, CREB and TrkB were measured by RT-PCT. RESULTS : 1. In the Noradrenalin content, RDM 100 and RDM 200 groups showed significant decrease compared to the control group. 2. In the GABA content, RDM 50 and RDM 100 groups showed significant decrease compared to the control group. 3. In the Melatonin content, RDM 100 and 200 groups showed significant decrease compared to the control group. 4. In the Activity of BDNF, RDM 100 and 200 groups showed significant increase compared to the control group. 5. In the Activity of CREB and TrkB, RDM 100 group showed significant increase compared to the control group. 6. In the Erythrocyte and Thrombocyte changes, Red blood cells and Hematocrit were significantly increased in RDM 50 and RDM 200 groups than the control group. Hemoglobin and platelet were significantly increased in all experimental groups and the control group. 7. In the Weight gain content, all RDM groups showed insignificant increase compared to the control group. 8. In the Glucose content, RDM 50, RDM 100, and RDM 200 groups showed significant decrease compared to the control group. CONCLUSION : These results suggest that RDM have effects on stress and sleep hormones when administered to Chronic Mild Stressed(CMS) rats.
more목차
ABSTRACT ⅰ
Ⅰ. 서론 1
Ⅱ. 재료 및 방법 3
1. 실험 재료 3
1) 실험 동물 3
2) 실험 약물 3
2. 실험 방법 5
1) Chronic Mild Stress(CMS) 유발 5
2) 실험군 분리 5
3) 약물 투여 5
4) 체중 및 사료효율 7
5) 혈액 및 혈청학적 검사 7
6) 혈당 측정 7
7) Immunohistochemistry ; Brain derived neurotrophic factor(BDNF)
측정 7
8) Reverse Transcription Polymerase Chain Reaction(RT-PCR) 8
9) ELISA에 의한 Noradrenaline 측정 10
10) ELISA에 의한 Gamma-Aminobutyric Acid(GABA) 측정 11
11) ELISA에 의한 Melatonin 측정 12
3. 통계처리 12
Ⅲ. 실험 결과 13
1. 체중변화에 미치는 영향 13
2. 혈당 및 혈청 Transaminase 함량에 미치는 영향 15
1) Glucose 15
2) AST 16
3) ALT 17
3. Leukocyte 변화에 미치는 영향 18
4. Erythrocyte 및 thrombocyte 변화에 미치는 영향 20
5. PCR 기법 ; BDNF, CREB, TrkB에 미치는 영향 22
1) BDNF 활성에 미치는 영향 22
2) CREB 활성에 미치는 영향 23
3) TrkB 활성에 미치는 영향 24
6. Noradrenalin 함량에 미치는 영향 25
7. GABA 함량에 미치는 영향 26
8. Melatonin 함량에 미치는 영향 27
Ⅳ. 고찰 28
Ⅴ. 결론 37
참고문헌 39